圈养大熊猫研究进展PDF电子书下载

第一部分　营养研究 2

饲喂纤维素酶喷洒的竹子提高病弱大熊猫养分消化率的初步试验 2

圈养亚成体大熊猫日粮粗蛋白表观消化率的初步研究　Study on the apparent digestibility of crude protein by sub-adult giant panda 4

圈养大熊猫乳汁营养成分分析 Nutrient content of the milk of captive giant pandas 9

大熊猫乳汁与牛、羊、人乳汁及人工乳中无机元素含量的比较研究 Comparison of inorganic elemen composition in milk of the giant panda,cow,goat,human artificial milk replace 16

大熊猫乳汁蛋白组成 Protein composition of the giant panda milk 19

圈养大熊猫主食竹的氨基酸分析 Study on the amino acids of bamboo ingested by captive giant panda 23

圈养大熊猫主食低山竹类营养特点的初步研究 Preliminary study on thye nutrient character of the stable food bamboo of captive giant panda 28

圈养大熊猫黏液排泄现象的观察 Preliminary observations on mucous excretion in the giant panda 33

圈养大熊猫受配后的行为研究 A study of the behavior of female giant pandas(Ailuropoda melanoleuca)following mating 38

大熊猫乳中酶的活力与激素含量分析　Enzyme activities and hormone concentrations in giant panda milk 45

大熊猫乳中富含游离精氨酸 Giant panda Ailuropoda melanoleuca milk contains high concentration of free arginine 48

大熊猫乳汁水解氨基酸含量的测定分析　Analysis of hydrolyzed amino acids in giant panda milk 54

Enzyme activities,hormone concentrations and protein composition in captive giant panda milk 59

Study of crude protein intake and growth response in captive subadult giant pandas(Ailuropoda melanoleuca) 63

Composition of captive giant panda milk 70

第二部分　繁殖育幼 76

首例大熊猫秋季发情、配种、产仔 Fall estrus and mating in a giant panda 76

圈养大熊猫同时哺育双胞胎行为观察 Observations on a captive giant panda nursing twin cubs 79

大熊猫妊娠及产仔行为观察 Observation on behaviors associated with gestation and parturition in giant pandas 86

大熊猫卵泡卵母细胞体外受精的初步研究 Preliminary study on fertilization in vitro of foll icular oocytes in giant panda(Ailurop oda melanoleuca) 89

大熊猫卵巢中“连体卵”的分离及其电镜观察 Isolation and electron microscopy of"twin oocytes"in the ovaries of giant panda(Ailuropoda melanoleuca) 93

大熊猫超轻初生幼仔人工哺育初探 Hand-rearing on newborn infant of low body we ight of giant panda(Ailuropoda melanleuca) 96

提高大熊猫幼仔存活率的研究 Study on improving the survival rate of infant giant panda(Ailuropoda melanleuca) 100

人工辅助大熊猫母兽哺乳幼仔研究 Humans assist a giant panda mother in rearing her baby 104

圈养大熊猫母兽成功哺育双胞胎成活初探 Successful rearing of twins by a female giant panda in captivity 109

利用阴道黏液电阻值进行大熊猫排卵监测的研究初报 A preliminary report on ovulation prediction using resistance value of vaginal mucus of giant panda(Ailuropoda melanoleuca) 114

繁殖期雌性大熊猫尿中激素变化的研究 Research on the changes of sex hormones in female giant pandas. Urine during estrus and gestation 117

大熊猫细管冻精制备程序的建立与应用 Development and application of new method for giant panda semen cryopreservation 122

大熊猫人工育幼研究 Artificial feeding of infant giant panda 126

大熊猫卵母细胞体外成熟及受精后的超微结构观察　Transmission electron-micrography of giant panda's oocytes after IVM and IVF 131

哺乳动物滋养层细胞在胚胎植入中的作用　The role of trophoblasts in mammalian embryo implantation 135

In vitro amturation of follicular oocytes of the giant panda ailuropoda melanoleuca a case report 140

Behavioral and developmental consequences of early rearing experience for captive giant pandas(Ailuropoda melanoleuca) 144

第三部分　生理生化 158

圈养大熊猫47项血液生理生化指标 158

大熊猫初乳和血清中免疫球蛋白的分离提纯及其抗血清的制备 The separation and purification of giant panda's immunoglobulins in colostrum and serum and the serumal preparation of rabbit anti-giant pandap's immunoglobulins 160

大熊猫胃肠道中消化酶活力的分析 163

大熊猫精清血小板激活因子乙酰水解酶（PAF-AH）活性影响因素的初步探讨 Preliminary studies on some factors which can influence platelet-activating factor acetylhydrolase(PAF-AH)activity in giant panda seminal plasma 166

大熊猫胃内纤毛虫检测初报 Preliminary report on ciliates found in stomach of giant panda Ailuropoda melanoleuca 170

不同EGF和胰岛素水平对体外培养的大熊猫皮肤成纤维细胞的影响 The effects the different concentration of EGF Epidermal Growth Factor and insulin on cutaneous fibroblast of giant panda(Ailuropoda melanoleuca)in vitro 174

圈养大熊猫黏液水解氨基酸含量的初步研究　Preliminary study on amino acids of mucus excreted from giant panda 180

大熊猫皮肤成纤维细胞系的建立和冷冻保存 Establishment and cryopreservation of a giant panda skin fibroblast cell line 185

大熊猫皮肤成纤维细胞在不同培养液中的生物学特性研究　Studies on biological characteristic of giant panda(Ailuropoda melanoleuca)skin fibroblasts in the different media 191

大熊猫皮肤组织酶解分离效果研究　Studies on enzyme digestion of cutaneous tissue of giant panda(Ailuropoda melanoleuca). 196

大熊猫肌肉氨基酸含量的初步研究　Study on the contents of amino acides in giant panda muscle 200

大熊猫初乳免疫球蛋白含量及其动态变化　Changes of immunoglobulins in colostrums of giant pandas 203

表皮生长因子和胰岛素对大熊猫体外培养皮肤成纤维细胞生物学特性的影响　Effects of epidermal growth factor and insulin on biological characteristics of giant panda(Ailuropoda melanoleuca)cutaneous fibroblast in vitro 208

圈养大熊猫排泄的黏液成分分析 Composition analysis of the muces excreted by captive giant panda 216

第四部分　遗传研究 222

应用微卫星分型方法进行大熊猫父亲鉴定 Paternity assignment of giant panda by microsatellite genotyping 222

圈养大熊猫的系谱分析　Pedigree analysis of captive giant panda 229

大熊猫生长激素受体（GHR）cDNA的克隆与序列分析　cDNA cloning and sequence analysis of growth hormone receptor(GHR)from giant panda　 236

大熊猫垂体泌乳素（PRL）cDNA的克隆与表达　Cloning and expression of pituitary prolactin gene in Ailuropoda melanoleuca 244

大熊猫IL-2基因的克隆与序列分析　Cloning and sequence analysis of the IL-2 gene in the giant panda Ailuropoda melanoleuca 251

Dynal磁珠富集大熊猫微卫星标记Enrichment of giant panda microsatellite markers using Dynal magnet beads 256

Molecular cloning of giant panda pituitary Prolactin cDNA and its expression in escherichia coli 262

Cloning and sequence analysis of FSH and LH in the giant panda(Ailuropoda melanoleuca) 269

cDNA cloning of Growth Hormone from giant panda(Ailuropoda melanoleuca)and its expression in Escherichia coli 277

A reliable,non-invasive PCR method for giant panda(Ailuropoda melanoleuca)sex identification 285

A novel genetic method for sex determination of giant panda 288

Di-,tri- and tetranucleotide microsatellite loci for the giant panda,Ailuropoda melanoleuca 292

第五部分　疾病防治 296

亚成体大熊猫金黄色葡萄球菌性呼吸道感染 296

大熊猫β-溶血性链球菌病的诊治 299

大熊猫吸入麻醉操作技术与监护方法 The use of gas anesthesia in giant pandas 300

大熊猫肝病变病例 307

大熊猫和小熊猫犬瘟热病与疫苗免疫现状 308

大熊猫慢性胃肠炎病研究 Study on the prevention and treatment of chronic gastroenteritis of giant pandas 314

大熊猫体表螨虫检出及处理 319

大熊猫顽固性胃肠功能紊乱伴严重厌食病例 320

佳灵三特-CA对大熊猫食皮螨病的治疗试验 322

大熊猫生殖道菌群研究　Study on the bacterial strain in the vagina of giant pandas 323

成都动物园大熊猫等珍稀哺乳动物寄生虫感染情况调查 326

微生态制剂在大熊猫疾病治疗中的临床应用研究 330

犬瘟热实验室诊断方法研究进展 333

降低圈养大熊猫排黏液频率的措施初探　Nutritional interventions to reduce mucus excretion in the captive giant panda(Ailuropoda melanoleuca) 338

大熊猫致病性催产克雷伯菌的分离鉴定　Isolation of Klebsiella oxytoca from giant panda 344

大熊猫幼仔口腔分泌物中检出聚团肠杆菌和微球菌　Isolation of E.agglomerans and Micrococcus from oral cavity secretion of a giant panda cub 347

大熊猫感染性泌尿生殖道血尿病原—肺炎克雷伯氏杆菌　Klebsiel a pneumoniae—pathogen in the urogenital tract of giant panda 350

大熊猫初生胎儿及幼仔组织器官的病理学研究　Pathological study on the organs of fetuses and cubs of giant panda 353

大熊猫幼兽腹泻粪便分离出的轮状病毒鉴定　Isolation and identification of rotavirus from giant panda cubs 358

首例圈养大熊猫难产的治疗与原因分析　Cause and treatment of the fisrt giant panda dystocia in captivity 364

第六部分　其他学科论文 372

成都大熊猫繁育研究基地夜鹭种群数量动态的观察 372

成都市区公共绿地野生鸟类调查初报 375

川金丝猴胰岛素样生长因子Ⅰ（IGF-Ⅰ）基因的克隆及序列分析 Cloning and sequence analysis of golden monkey(Rhinopithecus roxellana) insulin-like growth factor I gene 382

东北虎促卵泡激素和促黄体激素基因的克隆及其序列分析 Cloning and analysis of the homology of follicle-stimulating hormone and luteinizing hormone genes in the Siberican tiger(Panthera tigris al taica) 387

黑豹麻醉意外抽搐并休克抢救一例 394

华南虎、东北虎、孟加拉虎的D-loop和ND5序列及其在系统进化分析中的应用 Mitochondrial D-loop and nad h dehydrogenase subunit 5(ND5)gene of Panthera tigris amoyensis' P.Tigris altaica and P.Tigris tigris and their appl ication in phylogenetic analysis 396

佳灵三特-CA对灵长类动物内寄生虫的驱除试验 401

梅花鹿混合感染普通变形杆菌及大肠埃希氏菌的诊治 403

梅花鹿耶尔森氏菌感染的诊断和治疗 The diagnosis and treatment of Cervus nippon infected with Yersinia 405

山羊卵母细胞冷冻保存及其对发育效果的影响 Effect of cryopreservation on the developmental capacity of goat oocytes 407

山羊卵泡卵母细胞体外成熟体外受精研究 In vitro maturation and fertilization of goat oocytes 412

四川山鹧鸪脑源性神经营养因子（BDNF）基因的扩增与序列分析 The amplification and sequence analysis of the brain-derived neurotophic factor(BDNF)gene from Sichuan partridge 415

小鼠腔前卵泡的分离采集和体外成熟研究 Isolation and in vitro maturation of mouse preantral follicles 418

小熊猫寄生虫与寄生虫病研究进展 421

小熊猫圈养种群遗传多样性及其影响因子 Genetic diversity of the captive red panda population and its influencing factor 425

小熊猫肾脏和输尿管的组织学研究 Histological research on kidney and ureter of red pandas 428

小熊猫胃的解剖和组织结构研究　Study on anatomy and histology of stomach in red panda 432

亚洲黑熊电刺激采精及精液品质分析　Electroejaculation and semen characteristics of Asiatic black bears(Selena rctos thibetanus G.) 436

模拟自然生态的成都大熊猫繁育研究基地 439

两种高铬日粮对小鼠发育与健康的影响 Effects of two kinds of high level chrome diet on growth and health of mice 442

成都大熊猫繁育研究基地野生鸟类观察及其保护初探 444

麻醉对小鼠的排卵数量、排极率、存活率及孤雌激活率的影响 448

氯胺酮对PMSG-HCG诱发未成年小鼠排卵的影响 451

转sMTPGH基因小鼠胚胎的体外培养　In vitro culture of mouse embryos after pronuclear microinjection with sMTPGH fusin gene 453

Highly conserved D-loop-like nuclear mitochondrial sequences(NUMTS)in tiger(Panthera tigris)　 457

Primer note Isolation and characterization of 16 tetranucleotide microsatellite loci in the red panda(Ailurus fulgens) 467

Isolation and characterization of polymorphic tri-and tetra-nucleotide microsatellite loci for the South China tiger Panthera tigris amoyensis 470

PCR-CTPP:a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger(Panthera tigris)and four other endangered felids 474

Sequence variation of mitochondrial DNA ND5 in captive South China tigers(Panthera tigris amoyensis) 478

Twelve polymorphic microsatellite loci for the South China tiger Panthera tigris amoyensis 482

Introgression in captive South China tigers(Panthera tigris amoyensis) 485

Identification and characterization of ten polymorphic microsatellite loci in the red panda Ailurus fulgens 495