《Molecular biology and biotechnology: a guide for students Third Edition》PDF下载

  • 购买积分:15 如何计算积分?
  • 作  者:Helen Kreuzer
  • 出 版 社:ASM Press
  • 出版年份:2008
  • ISBN:1555814724
  • 页数:488 页
图书介绍:

PARTⅠ Laying the Foundation 1

1 An Overview of Biotechnology 3

2 Cell Properties and Processes 45

3 Genes,Genetics,and Geneticists 73

4 An Overview of Molecular Biology 97

5 Recombinant DNA Technology 137

PARTⅡ Classroom Activities 169

A.DNA Structure and Function 171

6 Student Activity:Constructing a Paper DNA Model 172

7 Student Activity:DNA Replication 174

8 Student Activity:From Genes to Proteins 176

Student Activity:Making Antisense:Regulating Gene Expression with RNA 179

9 Student Activity:Sizes of the Escherichia coli and Human Genomes 182

10 Student Activity:Extraction of Bacterial DNA 185

B.Manipulation and Analysis of DNA 187

11 Student Activity:DNA Scissors 188

12 Student Activity:DNA Goes to the Races 190

13 Student Activity:Restriction Analysis of Lambda DNA 194

14 Student Activity:Recombinant Paper Plasmids 199

15 Student Activity:Restriction Analysis Challenge 202

16 Student Activity:Detection of Specific DNA Sequences:PartⅠ.Fishing for DNA 205

Student Activity:Detection of Specific DNA Sequences:PartⅡ.Combining Restriction and Hybridization Analysis 207

Student Activity:Detection of Specific DNA Sequences:PartⅢ.Southern Hybridization 209

17 Student Activity:Paper PCR 210

18 Student Activity:DNA Sequencing:The Terminators 214

Reading:Chain Terminators as Antiviral Drugs 218

C.Transfer of Genetic Information 221

19 Student Activity:Transformation of Escherichia coli 222

20 Student Activity:Conjugative Transfer of Antibiotic Resistance in Escherichia coli 225

Reading:Gene Transfer and the Spread of Antibiotic Resistance 228

21 Student Activity:Transduction of an Antibiotic Resistance Gene 234

22 Student Activity:Agrobacterium tumefaciens:Nature’s Plant Genetic Engineer 238

D.Molecular Biology and Genetics 241

23 Student Activity:An Adventure in Dog Hair,Part Ⅰ 242

24 Student Activity:An Adventure in Dog Hair,Part Ⅱ:Yellow Labs 246

25 Student Activity:Human Molecular Genetics 250

Reading:Molecular Genetics of Cancer 258

26 Student Activity:Genetics in Action 264

E.Genomics 271

27 Student Activity:Comparing Genomes 272

Exercise 1:STRs Can Cause an RFLP 278

Exercise 2:PCR Can Reveal Differences at Microsatellite Loci 279

Reading:Mitochondrial DNA 281

28 Student Activity:Forensic DNA Typing 283

Exercise 1.A Mix-Up at the Hospital 285

Exercise 2.A Paternity Case 285

Exercise 3.The Case of the Bloody Knife 286

Reading:DNA Forensics:An Archaeological Case 289

29 Student Activity:Mapping a Disease Gene 290

Reading:The Human Genome Project:Its Science,Applications,and Issues 299

30 Student Activity:Microarray Analysis of Genome Expression 303

Reading:Personal Genomics 306

F.Bioinformatics and Evolutionary Analysis of Proteins 309

31 Student Activity:Testing for Amylase Activity 310

32 Student Activity:Electrophoresis of Amylase Samples 315

33 Student Activity:Constructing an Amylase Evolutionary Tree 318

34 Student Activity:Exploring Bioinformatics 322

PART Ⅲ Societal Issues 329

Introduction to Societal Issues 331

35 Student Activity:Facing the Forces:Science,Technology,and Society 332

Reading:The Nature of Science 338

36 Student Activity:Analyzing the Issues of Biotechnology:Cloning 341

Reading:Are Bioengineered Foods Safe? 352

37 Student Activity:The Risks of Technology:Perception,Reality,or Both? 356

Reading:Biotechnology Regulation in the United States 368

38 Student Activity:Making Decisions When There Is No Right Answer 372

39 Student Activity:Analyzing Ethical Dilemmas in Genetic Testing 377

Reading:Science,Law,and Politics 381

40 Student Activity:Analyzing Ethical Dilemmas in Gene Therapy 384

PART Ⅳ Appendixes 389

A Worksheets 390

B Laboratory Biosafety 453

C Aseptic Technique 455

Glossary 457

Index 469