Chapter 1 Basics of Physiological-Pharmacological Experimentation 1
Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment 1
Unit 2 Process of Experiment Data and Production of Experiment Reports 3
Unit 3 Requirements and Safety Rules in Laboratory 7
Unit 4 Operation of Biological Data Acquisition System RM-6240 15
Chapter 2 General Knowledge of Physiological-Pharmacological Experimentation 22
Unit 1 Fundamental Principle of Physiological-Pharmacological Experi-mentation 22
Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation 28
Unit 3 Statistical Analysis Most Often Used in Scientific Activities 31
Unit 4 Statistic Analysis Using Excel 35
Chapter 3 Basic Operations on Experimental Animals 44
Unit 1 Fundamentals of Animal Experiments 44
Unit 2 Operations on Experimental Animals 48
Unit 3 Gender Identification and Labeling the Animals 51
Unit 4 Narcotics and Animal Narcosis 55
Unit 5 Sample Collection and Bleeding 57
Unit 6 Drug Administration for Laboratory Animals 60
Chapter 4 Physiological-Pharmacological Experiments 66
Experiment 1 Generation and Conduction of Compound Action Potential 66
Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models 70
Experiment 2-1 Effect of Drugs on Electric Convulsive Shock 70
Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants 72
Experiment 3 Actions of Analgesics in Mouse Models 74
Experiment 3-1 Analgesic Effect Assayed by Hot-Plate Model 74
Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model 76
Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle 78
Experiment 5 Determination of ABO Blood Group 81
Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation 83
Experiment 7 Recording of Electrocardiogram in Toads 85
Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart 88
Experiment 9 Regulation of Artery Blood Pressure 91
Experiment 10 Measurement of Human Blood Pressure 94
Experiment 11 Toxic Effects of Cardiac Glycosides on Heart 97
Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles 101
Experiment 13 Regulation of Respiratory Movements 103
Experiment 14 Experimental Pulmonary Edema in Rabbits 106
Experiment 15 Factors and Drugs Influencing Urine Output 110
Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes 113
Experiment 17 Determination of 50 3.485388E-317ffective Dose of Pentobarbital 115
Experiment 18 Measurement of Concentration-Time Profile of Metronida-zole 118
Experiment 19 Calculation of the Pharmacokinetic Parameters 122
Experiment 20 Intoxication and Detoxification of Organophosphate Agents 127
Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice 130
Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model 133
Experiment 23 In-Vitro Experiment of Antibacterial Actions 136
Chapter 5 Expanded Experiments 139
Experiment 1 Extraction of Genomic DNA in Peripheral Blood 139
Experiment 2 Extraction of mRNA from Liver Tissues 142
Experiment 3 Assessment of Gene Transcription Level by Real-Time PCR 145
Experiment 4 Assessment of Protein Expression by Western-Blot 148
Experiment 5 Isolation and Culture of Mouse Hepatocytes 152
Appendix 156
Table 1 Physiological parameters of experimental animals 156
Table 2 Age,body weight and lifetime of adult experimental animals 157
Table 3 Formularies of commonly used saline solution 157
Table 4 Bleeding methods for experimental animals 158
Table 5 Safe and lethal bleeding volume of experimental animals 158
Table 6 Scaling factors for cross-species dose calculation 159
Table 7 Application route and dose of commonly used anaesthetics 160
参考文献 161