第一章 绪论 1
第一节 机能实验学教程的内容、目的和要求 1
第二节 实验报告的书写 2
第三节 实验室守则 4
第二章 机能学实验动物 5
第一节 实验动物的基本知识 5
第二节 实验动物的麻醉 12
第三节 实验动物用药量的确定 13
第三章 机能学实验常用仪器 15
第一节 微机技术与网络教学 15
第二节 RM6240C微机生物信号采集处理系统 15
第四章 机能学动物实验的基本操作技术 17
第一节 动物的捉拿及不同途径给药 17
第二节 坐骨神经—腓肠肌、坐骨神经干标本制备 24
第三节 离体蟾蜍心脏标本的制备 26
第四节 离体小肠段的制备 28
第五节 离体血管标本的制备 29
第六节 大鼠离体心脏Langendorff灌流技术 30
第七节 家兔活体解剖技术 31
第五章 机能学综合实验 34
第一节 刺激与肌肉收缩反应的关系 34
第二节 心电图描记，期前收缩和代偿间歇，离体蛙心灌流 36
第三节 局麻药对兔眼作用的观察 42
第四节 家兔动脉血压调节实验 43
第五节 水肿的观察及治疗 46
第六节 缺氧的观察及治疗 48
第七节 氨在肝性脑病发病中的作用 50
第八节 药代动力学实验 52
第九节 药物作用观察 60
第十节 不同途径给药观察及ED50测定 63
第十一节 家兔失血性休克 68
第十二节 家兔离体小肠段的特性观察及药物影响 70
第十三节 尿生成的调节及利尿药作用观察 72
第十四节 实验性急性右心衰 75
第十五节 病例讨论 79
参考文献 83
附录 84
一、常用实验溶液的成分及配制 84
二、常用实验动物的生理常数 84
Chapter 1 Introduction 88
Section 1 The Content, Purpose and Requirements of Functional Experiments course 88
Section 2 The writing of the experimental report 89
Section 3 The rules of laboratory 92
Chapter 2 Basic knowledge about laboratory animals required in the functional experiments 94
Section 1 Basic knowledge about laboratory animals 94
Section 2 Anesthesia of laboratory animals 102
Section 3 How to calculate the drug dosage to animals 104
Chapter 3 Common apparatus used in functional experiments 106
Section 1 Computer and local network technique used in teaching 106
Section 2 RM6240C channel physiological signal acquiring and processing system 106
Chapter 4 Basic operation technology onanimal experiment of functional science 109
Section 1 Animal handling and routes of administration 109
Section 2 Preparation of sciatic nerve-gastrocnemius muscle and sciatic nerve trunk samples 117
Section3 Preparation of isolated toad heart 119
Section 4 Preparation of isolated intestinal smooth muscle samples 122
Section 5 Preparation of isolated vascular specimens 123
Section 6 Langendorff perfusionof isolated rat heart 124
Section 7 Anatomical technique ofrabbits 125
Chapter 5 Comprehensive experiments of functional science 129
Section 1 The relationship between muscle stimulation and muscle contraction 129
Section 2 Recording electrocardiography, premature systole, compensatory pause and frog heart perfusion in vitro 132
Section 3 The surface anesthesia effect oftetracaine and procaine on rabbit eyes 139
Section 4 Functional experiments on blood pressure regulation in rabbits 141
Section 5 Observation and treatment of experimental edema in toads 145
Section 6 Observation and treatment of different hypoxia in mice 147
Section 7 Effects of ammonia on hepatic encephalopathy 150
Section 8 Drug metabolism and pharmacokinetics 153
Section 9 Therapeutic effects of drug on animal body 162
Section 10 Comparing the effects ofpentobarbital sodium on mice by different routes of administration and mensuration of ED50 166
Section 11 Rabbit model of acute hemorrhagic shock 171
Section 12 Analysis of effects of drugs acting on receptors of the rabbit isolated intestinal smooth muscles 174
Section 13 Regulation of urine production and effect of diuretic in rabbits 177
Section 14 Acute right heart failure in rabbits and the therapeutics 181
Section 15 Case discussion 184
References 190
Appendix 192
1. Preparation and Component of Experimental Solution 192
2. Physiological Constants of Laboratory Animals 192