1 氮素循环和生物固氮（BNF）、生物固氮的经济和生态效益 1
2 固氮微生物 6
3 根瘤菌与豆科植物的共生 6
4 结瘤过程 11
5 大豆根瘤菌及其在中国的多样性 14
6 中国的大豆栽培 17
7 接种的有效性 22
8 接种剂生产 25
8.1 对接种需求的评定 25
8.2 商业接种剂的类型 27
8.3 接种剂的载体及其灭菌 29
8.4 包装 34
9 根瘤菌接种剂的质量控制（QC） 34
10.1 从根瘤中分离根瘤菌菌株 38
10 菌株筛选 38
10.2 从根瘤分离物鉴定根瘤菌菌株 40
10.2.1 植物试验和共生参数的评价 40
10.2.2 用于鉴定根瘤菌菌株的遗传学特征 42
11 竞争结瘤与鉴别根瘤菌菌株的方法 44
11.1 培养特征 45
11.2 标记系统 46
12 培养基 50
12.1 细菌培养基 50
12.2 结瘤试验所用的植物营养溶液 54
13 根瘤菌菌种的保存 56
14 田间试验 58
15 最大概率数（MPN）法估算土著根瘤菌种群 60
16 有用的地址 64
参考文献 66
Introduction 88
1 The Nitrogen Cycle and Biological Nitrogen Fixation(BNF)Economic and ecological advantages of BNF 90
2 Nitrogen-fixing microorganisms 96
3 The Rhizobium-legume symbiosis 98
4 The nodulation process 102
5 Soybean microsymbionts and their diversity in China 107
6 Soybean cultivation in China 110
7 Effectiveness of inoculation 118
8 Inoculant production 120
8.1 Assessing the need for inoculation 120
8.2 Types of commercial inoculants 122
8.3 Inoculant carriers. Sterilization of carriers 125
8.4 Packaging 131
9 Quality Control(QC)of rhizobial inoculants 132
10 Strain Selection 135
10.1 Isolation of rhizobial strains from nodules 136
10.2 Authentication of nodule isolates as rhizobial strains 138
10.2.1 Plant testing and evaluation of symbiotic parameters 138
10.2.2 Genetic traits used to identify rhizobial strains 142
11 Competition for nodulation. Methods for identifying rhizobial strains 144
11.1 Growth characteristics 145
11.2 Marker systems 147
12 Culture Media 152
12.1 Bacterial culture media 152
12.2 Plant nutrient solutions for nodulation assays 157
13 Preserving rhizobial cultures 158
14 Field Experiments 161
15 Most Probable Number(MPN)method for estimating the indigenous rhizobia population 164
16 Useful addresses 168
References 170