Chapter 1 Implementation Guide 2
How to Use This Book 2
Teaching Strategies 4
Assessment 6
How to Start a Biotechnology Course 7
Independent Research Projects 10
Chapter 2 Laboratory Skills 12
Activity 2.1 DNA Extraction and Precipitation 12
Activity 2.2 Pipetting 16
Activity 2.3 Kool-Aid Column Chromatography 19
Activity 2.4 Making Solutions 23
Activity 2.5 Titration 28
Activity 2.6 Writing a Standard Operating Procedure 31
Chapter 3 Microbiology and Cell Culture 34
Activity 3.1 Making Microbiology Media 34
Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test） 40
Activity 3.3 Microbes and Health: An Illustration ofKoch's Postulates 44
Activity 3.4 Gram Staining 49
Activity 3.5 Quantifying Bacterial Numbers 52
Activity 3.6 Staining Eukaryotic Cells 55
Chapter 4 DNA Structure and Analysis 58
Activity 4.1 Restriction Site Prediction Using NEBcutter 58
Activity 4.2 Casting Agarose Gels 61
Activity 4.3 Dye Electrophoresis 65
Activity 4.4 Restriction Digestion and Analysis of Lambda DNA 69
Activity 4.5 Forensic DNA Fingerprinting 75
Activity 4.6 Plasmid Mapping 80
Chapter 5 Bacterial Transformation & Plasmid PurificationActivity 5.1 Bacterial Transformation with S3 Plasmid 84
Activity 5.2 Bacterial Transformation with pGLO Plasmid 89
Activity 5.3 Purification of S3 and pGLO Plasmids 93
Activity 5.4 DNA Quantitation 97
Chapter 6 The Polymerase Chain Reaction 102
Activity 6.1 STR PCR Analysis 102
Activity 6.2 GMO Detection by PCR 107
Activity 6.3 Detection of the Human PV92 Alu Insertion 114
Chapter 7 Protein Structure and Analysis 120
Activity 7.1 Protein Quantitation Using the Bradford Assay 120
Activity 7.2 Size Exclusion Chromatography 124
Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography 127
Activity 7.4 SDS-PAGE of Fish Muscle 132
Activity 7.5 Biofuel Enzyme Assay 137
Activity 7.6 Exploring Bioinformatics with GFP 143
Chapter 8 Immunological Applications 148
Activity 8.1 Ouchterlony Double Immunodiffusion Assay 148
Activity 8.2 Serum Antibody Detection by ELISA 152
Activity 8.3 Quantitative ELISA 156
Activity 8.4 Western Blotting 160
Appendices 167
Appendix Ⅰ: Debate Activity 167
Appendix Ⅱ: Technical Writing Rubric 169
Appendix Ⅲ: Semilog Graph Paper 170
Appendix Ⅳ: Activities Sorted By Skill Level 171
Appendix Ⅴ: Pacing Guides 172
Appendix Ⅵ: Purchasing Guide 174
Appendix Ⅶ: Trademarks and Legal Notices 187