PART Ⅰ Introduction 1
1 A Brief History 1
2 The Molecular Nature of Genes 16
3 An Introduction to Gene Function 38
PART Ⅱ Methods in Molecular Biology 59
4 Molecular Cloning Methods 59
5 Molecular Tools for Studying Genes and Gene Activity 91
PART Ⅲ Transcription in Prokaryotes 133
6 The Transcription Apparatus of Prokaryotes 133
7 Operons:Fine Control of Prokaryotic Transcription 175
8 Major Shifts in Prokaryotic Transcription 205
9 DNA-Protein Interactions in Prokaryotes 231
PART Ⅳ Transcription in Eukaryotes 261
10 Eukaryotic RNA Polymerases and Their Promoters 261
11 General Transcription Factors in Eukaryotes 297
12 Transcription Activators in Eukaryotes 342
13 Chromatin Structure and Its Effects on Transcription 380
14 Posttranscriptional EventsⅠ:Splicing 418
PART Ⅴ Posttranscriptional Events 418
15 Posttranscriptional EventsⅡ:Capping and Polyadenylation 462
16 Posttranscriptional EventsⅢ:Other Events 497
PART Ⅵ Translation 528
17 The Mechanism of TranslationⅠ:Initiation 528
18 The Mechanism of TranslationⅡ:Elongation and Termination 567
19 Ribosomes and Transfer RNA 606
20 DNA ReplicationⅠ:Basic Mechanism and Enzymology 642
PART Ⅶ DNA Replication,Recombination,and Transposition 642
21 DNA ReplicationⅡ:Detailed Mechanism 684
22 Homologous Recombination 717
23 Site-Specific Recombination and Transposition 747
PART Ⅷ Genomes 784
24 Genomics 784
Glossary 819
Index 842