Chapter 1. An Introduction of Functional Experimentation 1
Chapter 2. Basic Knowledge and Theory 7
Section 1. Biological Characteristics and Application of Commonly Used Laboratory Animals 7
Section 2. Biological Data Acquisition Systems 13
Section 3. Selection and Design Principles of Medical Scientific Research Topics 20
Section 4. Medical Information Retrieval 27
Section 5. Writing Methods of Scienti-fic Papers and Lab Reports 30
Section 6. Laboratory Safety and Rules 35
Chapter 3. Basic Methods of Animal Experiments 43
Section 1. Animal Capture and Restraint 43
Section 2. Animal Numbering 46
Section 3. Drug Administration for Laboratory Animals 48
Section 4. Anesthesia of Laboratory Animals 52
Section 5. Unhairing of Laboratory Animals 57
Section 6. Blood Collection of Laboratory Animals 58
Section 7. Euthanasia Methods of Laboratory Animals 65
Chapter 4. Basic Techniques in Experimental Animal Surgical Operation 67
Section 1. Traditional Surgical Instruments 67
Section 2. Technique of Animal Catheteriz ation 69
Section 3. Haemostasis and Technique of Knots Making 72
Section 4. Technique of Wound Sutures （Stitches）and Sutures Out 74
Chapter 5. Basic Experiments 76
Experiment 1. The Nature of Muscle Contraction 76
Experiment 2. Measurement of Conduction Velocity of Action Potential and Excitability from Neural Trunk 78
Experiment 3. Determination of Hemogl-obin Content and Red Blood Cell Osmotic Fragility 82
Experiment 4. Premature Contraction and Compensatory Pause 84
Experiment 5. Chemicals on the regulation of ex vivo heart activities 86
Experiment 6. Measurement of Human Arterial Blood Pressure 88
Experiment 7. Recording of Human Ele- ctrocardiogram 91
Experiment 8. Pulmonary Function Tests 93
Experiment 9. Regulation of Respiratory Movements 96
Experiment 10. Physiological Properties of the Isolated Gastrointes- tinal Smoth Muscle 98
Experiment 11. Factors That Influencing Urine Formation 100
Experiment 12. Damage Effects of Unilat eral Labyrinth and Cochlear Potential Induction from Guinea Pigs 103
Experiment 13. Examination of Visual Fields and Scotomas 105
Experiment 14. Analysis of Reflex Arc and the Basic Features of the Reflex Activities 107
Experiment 15. Experimental Pulmonary Edema in Rabbits 111
Experiment 16. Effect of Different Factors on Hypoxic Tolerance 113
Experiment 17. Influence of pH of Body Fluids on Drug Absorption 114
Experiment 18. One-Compartment Open Model and Calculation of the Pharmacokinetic Parameters 115
Experiment 19. Two-Compartment Open Model and Calculation of the Pharmacokinetic Para-meters 119
Experiment 20.Determination of Medial Lethal Dose(LD50)of Drug 122
Experiment 21.The Antergic Experiments of Drug—the Binding Assay of Radio-Ligand and Receptor 123
Experiment 22.Intoxication and Detoxication of Organophosphates 126
Experiment 23.Effects of Drugs by Differ-ent Dosage Forms 128
Experiment 24.Effects of Drugs by Differ-ent Routes of Administrat-ion 130
Experiment 25.Basic Effects of Drugs 131
Experiment 26.Factors Influencing Drug Effects 133
Experiment 27.Observation of Analgesic Action of Drugs with Wri; thing Method 136
Experiment 28.Anticonvulsant Effect of the Drug 137
Experiment 29.Effects of Drugs on Lear- ning andMemory ofAnimal (Mice step-down experiment, rat shuttle box expenment, morris watermaze experiment) 139
Experiment 30.Effects of Hydrocortisone on Mice Ear Swelling In- duced by Xylol 144
Experiment 31. Anti inflammatory Effects of Dexamethasone on Rat Paw Edema 145
Experiment 32. Effects of Cardiac Glycosides on Heart Failure 147
Experiment 33. Antibacterial Experiment of Antibacterial Agents in Vitro 150
Experiment 34. Antibacterial Experiment of Antibacterial Agents in Vivo 152
Chapter 6. Comprehensive Experiments 157
Experiment 1. Different Types of Hypoxia 157
Experiment 2. Hyperkalemia in Rabbits 159
Experiment 3. Disseminated Intravascular Coagulation in Rabbit 161
Experiment 4. Disorders of Acid-Base Bal-ance and Its Experimental Therapy in Rabbits 166
Experiment 5. Ischemia and Reperfusion Injury in Intestine 170
Experiment 6. Acute Endotoxic Shoek 172
Experiment 7. Hemorrhagic Shock and Ah-erations of Microcirculation in Rabbit 175
Experiment 8. Regulation of the Cardiovasc-ular System and Acute Card-iac Failure 178
Experiment 9. Effects of Experimental Pn-eumothorax on Respiration, Circulation and Acid-Base Balance in Rabbits 183
Experiment 10. Acute Respiratory Distress Syndrome and Pharmacoth- erapy 186
Experiment 11. Hepatic Encephalopathy and Its Treatment 189
Experiment 12. Determination of Endogen-ous Creatinine Clearance Rate and Excretion Fraction of Sodium Filtration in Acute Renal Failure 192
Experiment 13. Reproduction of Multiple Organ Dysfunction Syndr-ome 193
Experiment 14. The Effect of Drug and Bio-activator on the Isolated Tracheal Strips of Cavy 198
Experiment 15. Determination of the Phar-macokinetic Parameters of Sulfa Drugs in Rabbits with Renal Failure 200
Chapter 7. Exploratory Experiments 204
Chapter 8. Case Discussion and Paper Analysis 205
References 239
Appendix 240