《植物生理分析技术》PDF下载

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  • 作  者:高景辉著
  • 出 版 社:五南图书出版股份有限公司
  • 出版年份:2005
  • ISBN:9571139378
  • 页数:160 页
图书介绍:

前言 1

第一部分:化学成分分析 3

Abscisic acid(ABA) 4

Al 11

Amino acids 14

Amino nitrogen 15

Anthocynins&flavonoids and/or sinapate esters 17

Ascorbate and dehydroascorbate 18

Cd 20

Chlorophyll 23

Cu 25

Fe 28

Glutathione(GSH&GSSG) 31

Glycinebetaine 34

Hydrogen peroxide(H202) 36

Lignin 38

Malondialdehyde(MDA) 41

Na 43

Ni 46

NH4+ 49

N03- 52

Pi(无机磷) 54

Polyamines 56

Proline 60

Protein 62

Reducing sugar 64

Relative water content(RWC) 67

Starch 68

Total peroxide 71

Total sugars 72

Transpiration rate 74

第二部分:酵素分析 75

Acid phosphatase(APase) 76

Adenosine triphosphatase(ATPase) 79

a-Amylase 81

Ascorbate oxidase 83

Ascorbate peroxidase 85

Catalase 87

Diamine oxidase 89

Glutamate dehydrogenase(GDH) 91

Glutamine sythetase(GS) 93

Glutathione reductase(GR) 96

Glycolate oxidase 98

Inorganic pyrophosphatase(IPPase) 100

Ornithine aminotransferase(OAT) 105

Peroxidase 107

Phenylalanine ammonia-lyase(PAL) 110

Proline dehydrogenase 112

Protease(Ⅰ) 114

Protease(Ⅱ) 116

△1-Pyrroline-5-carboxylate reductase(P5CR) 122

Superoxide dismutase(SOD) 124

Syringaldazine peroxidase 126

Wall-bound ferulate-peroxidase 128

Wall-bound NADH-peroxidase 130

Wall-bound peroxidase 133

第三部分:电泳分析 137

Acid phosphatase(APase)isozyme:Native PAGE 138

Peroxidase isozyme:Isoelectric focusing(IEF)electrophoresis 141

SDS-PAGE:Coomassie brilliant blue stain(Sodium dodecylsulfate polyacrylamide gel electrophoresis) 144

Superoxide dismutase(SOD)isozymes 148

附录 153

水稻幼苗之水耕栽培法 154

常用试剂原液之配制 156

各种缓冲溶液及其最适pH值范围 159

PEG6000溶液与渗透势换算表 160