前言 1
第一部分:化学成分分析 3
Abscisic acid(ABA) 4
Al 11
Amino acids 14
Amino nitrogen 15
Anthocynins&flavonoids and/or sinapate esters 17
Ascorbate and dehydroascorbate 18
Cd 20
Chlorophyll 23
Cu 25
Fe 28
Glutathione(GSH&GSSG) 31
Glycinebetaine 34
Hydrogen peroxide(H202) 36
Lignin 38
Malondialdehyde(MDA) 41
Na 43
Ni 46
NH4+ 49
N03- 52
Pi(无机磷) 54
Polyamines 56
Proline 60
Protein 62
Reducing sugar 64
Relative water content(RWC) 67
Starch 68
Total peroxide 71
Total sugars 72
Transpiration rate 74
第二部分:酵素分析 75
Acid phosphatase(APase) 76
Adenosine triphosphatase(ATPase) 79
a-Amylase 81
Ascorbate oxidase 83
Ascorbate peroxidase 85
Catalase 87
Diamine oxidase 89
Glutamate dehydrogenase(GDH) 91
Glutamine sythetase(GS) 93
Glutathione reductase(GR) 96
Glycolate oxidase 98
Inorganic pyrophosphatase(IPPase) 100
Ornithine aminotransferase(OAT) 105
Peroxidase 107
Phenylalanine ammonia-lyase(PAL) 110
Proline dehydrogenase 112
Protease(Ⅰ) 114
Protease(Ⅱ) 116
△1-Pyrroline-5-carboxylate reductase(P5CR) 122
Superoxide dismutase(SOD) 124
Syringaldazine peroxidase 126
Wall-bound ferulate-peroxidase 128
Wall-bound NADH-peroxidase 130
Wall-bound peroxidase 133
第三部分:电泳分析 137
Acid phosphatase(APase)isozyme:Native PAGE 138
Peroxidase isozyme:Isoelectric focusing(IEF)electrophoresis 141
SDS-PAGE:Coomassie brilliant blue stain(Sodium dodecylsulfate polyacrylamide gel electrophoresis) 144
Superoxide dismutase(SOD)isozymes 148
附录 153
水稻幼苗之水耕栽培法 154
常用试剂原液之配制 156
各种缓冲溶液及其最适pH值范围 159
PEG6000溶液与渗透势换算表 160