CHAPTER ONE INTRODUCTION 1
Section 1 The Content,Purpose and Requirements of Functional Experiments Course 1
1.1.1 Content 1
1.1.2 Purpose 1
1.1.3 Requirements 1
1.1.4 Format of Experiment Report 3
Section 2 Basic Knowledge Required in the Functional Experiments 4
1.2.1 Basic Knowledge about Laboratory Animals 4
1.2.2 How to Calculate the Drug Dosage to Animals 11
1.2.3 Animal Handling and Different Routes of Administration 12
1.2.4 Anesthesia of Laboratory Animals 19
Section 3 Common Apparatus Used in Functional Experiments 20
1.3.1 RM6240C Channel Physiological Signal Acquiring and Processing System 20
1.3.2 BL-420 Data Acquisition and Analysis System for Life Science 38
1.3.3 BI-2000 System of Image Processing and Analysis 49
1.3.4 Computer and Local Network Technique Used in the Teaching 51
CHAPTER TWO BASIC EXPERIMENTS IN FUNCTIONAL EXPERIMENTS 53
Section 1 Analog Experiments on the Computer or Machine 53
2.1.1 Analog Experiments of Electro-physiology of the Calamary's Huge Axon on the Computer 53
2.1.2 Analog Experiments of Mechanics of Muscle Contraction on the Computer 55
2.1.3 Analog Experiments of One Compartment Open Model on the Machine 58
2.1.4 Analog Experiments of Two Compartment Open Model on the Machine 60
Section 2 Functional Experiments on Isolated Preparations 63
2.2.1 Preparation of Sciatic Nerve-Gastrocnemius(SNG)Samples 63
2.2.2 Influences of the Muscle Contraction through Different Stimulation Intensities 64
2.2.3 Influences of the Muscle Contraction through Different Stimulation Frequencies 65
2.2.4 Preparation of Isolated Toad Heart 66
2.2.5 Observation and Recording of Toad's Heart Beat 68
2.2.6 The Effects of Electrolyte on the Heart Activity 69
2.2.7 The Physiological Characters of the Rabbit Small Intestine Segment in-vitro 70
2.2.8 The Influence of Drugs on Isolated Toad Heart 72
2.2.9 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle of a Rabbit 75
Section 3 Functional Experiments on Intact Preparations 78
2.3.1 The Discharge of Rabbit Depressor Nerve 78
2.3.2 Factors that Affect the Urine Formation in a Rabbit 80
2.3.3 Comparing the Effects of Pentobarbital Sodium on Mice by Different Routes of Administration 82
2.3.4 The Surface Anesthesia Effect of Tetracaine and Procaine on Rabbit Eyes 83
2.3.5 The Epidural Anesthesia Effect of Procaine on Rabbits 85
2.3.6 Mensuration Dose-effect Curve of NA on Blood Pressure in Rabbits 86
2.3.7 Effect of Chlorpromazine on Body Temperature Regulation in Rats 89
CHAPTER THREE INTEGRATED AND ADVANCED EXPERIMENTS IN FUNCTIONAL EXPERIMENTS 90
Section 1 Multi-physiological Index Observation on Animal Body 90
3.1.1 Nervous and Humoral Regulation of Arterial Blood Pressure in a Rabbit 90
3.1.2 The Stimulus Effects on the Motor Area in Pallium of a Rabbit 93
3.1.3 Nervous Regulation of the Respiratory Movement in a Rabbit 94
3.1.4 Mensuration of ED50 of Sodium Pentobarbital in Mice 95
3.1.5 Effects of Drugs on Cardiovascular System of a Rabbit 98
Section 2 Preparation of Animal Disease Model 100
3.2.1 A Rabbit Model for Hyperkalemia 100
3.2.2 An Analysis of Experimental Edema in Toads 103
3.2.3 Characterization of the Different Types of Hypoxia 105
3.2.4 A Rabbit Model of Acute Hemorrhagic Shock 107
3.2.5 The Effects of Ammonia on Hepatic Encephalopathy 109
Section 3 Therapeutic Effects of Drugs on Animal Body 111
3.3.1 Intoxication and Detoxication of Organophosphorus 111
3.3.2 Anticonvulsant Effect of Drugs in Mice 112
3.3.3 Observation of Analgesic Action of Drugs with Turning Trunk Method in Mice 113
3.3.4 Toxic Effect of Streptomycin Sulfate and Antagonistic Effect of Calcium Chloride on a Rat 115
3.3.5 Diuretic Effect of Drugs on a Rabbit 116
3.3.6 Effect of Glucocorticoids on Permeability of Capillary Vessels in Mice 118
CHAPTER FOUR EXAMINATION ITEMS IN FUNCTIONAL EXPERIMENTS 119
Section 1 Observation and Preparation of Isolated Animal Samples 119
4.1.1 Observation and Recording of Toad's Heart Beat 119
4.1.2 Preparation of Sciatic Nerve-Gastrocnemius(SNG)Samples 121
Section 2 Analysis and Preparation of Animal Disease Model 122
4.2.1 A Rabbit Model of Acute Hemorrhagic Shock 122
4.2.2 Nervous and Humoral Regulation of Arterial Blood Pressure in a Rabbit 124
4.2.3 Factors that Affect the Urine Formation 127
Section 3 An Analysis of Effects of Different Drugs on Animals or Samples 129
4.3.1 Effect of Drugs on Receptors in Isolated Intestine Smooth Muscle 129
4.3.2 Analysis of the Effects of Drags Acting on the Central Nerve System 133
APPENDIX 134
Preparation and Component of Experimental Solution 134
Physiological Constants of Laboratory Animals 134
REFEENCE 135