1 Agarose and Polyacrylamide Gel Electrophoresis&Andrea M. Guilli 1
2 Internal Labeling of DNA Probes&Ralph Rapley and Bimal D. M. Theophilus 13
3 End-Labeling of DNA Probes&Adrian J. Harwood 17
4 Southern Blotting of Agarose Gels by Capillary Transfer&Ralph Rapley and lan J. Williams 23
5 Restriction Fragment Length Polymorphism&Mohammad S. Enayat 29
6 PCR: Principles, Procedures, and Parameters&Nicola Louise Jones 37
7 Allele-Specific Oligonucleotide PCR&Elaine K. Green 47
8 Long-Range PCR&Peter A. Davies and George Gray 51
9 Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing&Anu Wartiovaara and Ann-Christine Syv？nen 57
10 Cycle Sequencing of PCR Products&G. K. Surdhar 65
11 Fluorescent In Situ Hybridization&Sara A. Dyer and Elaine K. Green 73
12 The Protein Truncation Test&Carol A. Hardy 87
13 Mutation Detection in Factor Ⅷ cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch&Naushin H. Waseem, Richard Bagnall, Peter M. Green,and Francesco Giannelli 109
14 Denaturing Gradient Gel Electrophoresis&Yvonne Wallis 125
15 Conformation-Sensitive Gel Electrophoresis&lan J. Williams and Anne C. Goodeve 137
16 SSCP/Heteroduplex Analysis&Andrew J. Wallace 151
17 Cleavase？ Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection&Laura Heisler and Chao-Hung Lee 165
18 Automated Genotyping Using the DNA MassArrayTM Technology&Christian Jurinke, Dirk van den Boom, Charles R. Cantor,and Hubert K？ster 179
19 An Introduction to Bioinformatics&Henry Brzeski 193
Index 209