第五届国际转基因动物学术研讨会论文集PDF电子书下载

SECTION 1 TRANSGENIC ANIMALS AND MAMMARY GLAND BIOREACTOR（转基因动物与乳腺生物反应器） 1

0101 CONSTRUCTSYNTHETICGENEENCODINGARTIFICIALSPIDERDRAGLINESILKPROTEINANDITS EXPRESSION IN MILK OF TRANSGENIC MICE&Xu Hong-Tao,Fan Bao-Liang,Yu Shu-yang等 1

0102 SUPERTRANSGENIC CARP:PREPARATION OF GYNOGENETIC OFFSPRING AND ANALYSIS OF GENOTYPIC PURITY&LING Li-Qun(梁利群)，YAN Xue-Chun(闫学春)，SUN Xiao-Wen(孙效文)等 6

0103 THE ESTABLISHMENT OF TRANSGENIC MICE OF HUMAN PROTEIN C GENES AND THE GENE'S EXPRESSION IN MAMMARY GLAND（人蛋白C转基因小鼠的建立及乳腺表达）&ZHAO Bao-quan(赵宝全)，ZHANG Jin-Gang(章金刚)等 9

0104 EXPRESSION OF TRANSGENIC MICE CARRYING GREEN FLUORESCENT PROTEIN GENE（绿色荧光蛋白基因（GFP）在转基因小鼠体内的表达）&XU Yi-mei(徐艺玫)，LIU Li-Jun(刘丽均)等 12

0105 THE ESTABLISHMENT AND APPLICATION OF THE NUCLEAR TRANSPLANTATION OF MAMMALIAN EGG CELL—NEW RESEARCH ON THE TECHNIQUE OF HUMAN EMBRYONIC STEM CELLAND TRANSGENIC ANIMAL（哺乳动物卵细胞核移植技术的建立及应用—人类胚胎干细胞及转基因动物技术新探）&XIONG Ya-Long(熊亚隆)，NA Dong Quan(纳冬荃)，YUE Yu-Han(岳玉罕) 15

0106 GENE PACKED USING PEI IN COMBINATION WITH ELECTROPORATION FACILITATE THE EXOGENOUS DNA ENTRY INTO THE NUCLEUS OF MOUSE SPERM（阳离子多聚物PEI结合电穿孔实现小鼠精子的核内转染的研究）&HUANG Wu-Jian（黄吴键），YUAN Jin（袁进）等 18

0107 EXPERIMENTAL STUDY ON GENERATION OF TRANSGENIC MICE BY COMBINATION OF INTRATESTICULAR INJECTION WITH IN VIVO ELECTROPORATION（睾丸内注射和体内电穿孔联合法建立转基因小鼠的实验研究）&YUAN Jin（袁进），AN Jing(安靓)等 22

0108 PRODUCTION OF TRANSGENIC MICE CARRYINGHUMAN P16INK4A BY MICROINJECTION（用显微注射法制备携带人P16基因的转基因小鼠的初步研究）&ZHANG Shou-Chun（张守纯），YU Ye-Hui（于业辉）等 27

0109 STUDIES ON THE MECHANISM OF THE STABLEEXPRESSION OF EXOGENOUS GENE IN MAMMARY GLANDS（乳腺持续表达外源基因的机理研究）&AN Jing(安靓)，LI Jin（李进）等 29

0110 ESTABLISHMENT OF TRANSGENIC MOUSE MODEL WITH LYMPHOMA&GUO Chang-Zhan，ZHOU Wei-Min等 31

0111 ANIMAL CLONING AND TRANSGENIC MAMMARY GLAND BIOREACTORS（动物克隆与转基因动物乳腺生物反应器）&Cheng Guo-Xiang（成国祥） 32

0112 VISUALIZING TUMOR DEVELOPMENT AND GENE EXPRESSION IN VIVO THROUGH BIOLUMINESCENCE IMAGING&Ning Zhang 32

0113 GENERATION OF MT-PA TRANSGENIC GOAT BY SPERM-MEDIATED GENE TRANSFER（利用精子载体方法生产MT-PA转基因山羊）&叶华虎，刘珠果，卢建申等 33

0114 APRELIMINARY STUDY ON GENERATION OF TRANSGENIC ANIMALS BY INTRA-OVARY INJECTION（卵巢注射法制备转基因动物的初步研究）&刘维全，王吉贵，李全等 33

0115 BIOLOGICAL CHARACTERISTICS OF THE TRANSGENIC MOUSE HARBORING HBV COMPLETE GENOMIC DNA（乙型肝炎病毒全基因组转基因小鼠的生物学特性）&ZI Xiao-Yuan(訾晓渊)，ZHANG Nan（张南）等 34

0116 CRUCIAL ROLE OF GENE ORDER IN THE DEVELOPMENTALLY REGULATED EXPRESSION MODE OF HUMAN α-GLOBIN GENE CLUSTER&WANG Yi，WANG Zhao等 36

0117 BOTHHS-40 AND HS-4～33 ARE SIGNIFICANT FOR THE REGULATION OF THEHUMAN α-LIKE GLOBIN GENE EXPRESSIONS ON THE WHOLE CLUSTER LEVEL&TANG Xiao-Bin，FENG Dong-Xiao等 37

0118 EXPLORING THE FUNCTION OF PSEUDOGENES ON THE HUMAN ALPHA GLOBIN CLUSTER&ZHAO Wang，KE Xi-Song等 37

0119 CREATING TRANSGENIC MODEL OF CARDIAC DISEASES WITH C57BL/6J MICE&ZHUO Ming-Lei，LIU Guang等 38

0120 MEASUREMENT OF BLOOD PRESSURE O FTRANSGENIC C57BL/6J MICE MODEL OF CARDIAC DISEASES&SUN Li-Hong,ZHOU Ming-Lei等 38

0121 CONSTRUCTION OF RED FLUORESCENCE PROTEIN TRANSGENIC WHITE CLOUD MOUNTAIN MINNOW&JIAN Qing,BAI Jun-Jie等 39

0122 ANIMAL MODEL FOR REAL-TIME MEASUREMENTS OF NEURONAL ACTIVITY IN TRANSGENIC MICE&Shi-Liang Shen,Allsion Xu等 40

0123 INDUCIBLE REPORTER AND HIV NEF GENE EXPRESSION IN CD4+CELLS OF TRANSGENIC MICE&Jisen Huai,Gabi Niedenmann 40

0124 OVEREXPRESSION OF GLY56/GLY80/GLY81-MUTANT INSULIN-LIKE GROWTH FACTOR（IGF）BINDING PROTEIN-3（IGFBP-3）IN TRANSGENIC MICE&GUI Yao-Ting,Josef V.Silha等 41

0125 HIGH EXPRESSION OF HUMAN ERYTHROPOIETIN IN MAMMARY GLAND OF TRANSGENIC GOATS&CHEN Jian-Quan,WU Guo-Xiang等 42

0126 EXPRESSION OF AN ENGINEERED FORM OF HUMAN TISSUE-TYPE PLASMINOGEN ACTIVATOR IN MOUSE MILK&SHA Hong-Ying（沙红英），LIU Si-Guo（刘思国）等 42

0127 EFFICIENT GENERATION OF TRANSGENIC GOATS BY NUCLEAR TRANSFER&YU Guo-Hhua(余国华),WU Guo-Xiang（吴国祥）等 43

0128 TRANSFER OF AN EXPRESSION YAC INTO GOAT FETAL FIBROBLASTS BY CELL FUSION FOR MAMMARY GLAND BIOREACTOR&ZHANG Xu-Ffeng,WU Guo-Xiang等 43

0129 THE CHARACTERIZATION AND BIOLOGIC ACTIVITY OF RECOMBINANT HUMAN ERYTHROPOIETIN IN GOAT MILK&ZHAO Jian-Yang，CHEN Jian-Quan等 44

0130 GENERATION OF BOVINE FCRN ALPHA CHAIN HARBORING BAC TRANSGENIC MICE&Bender B.,Bodrogi L.等 44

0131 IMPROVING EFFICIENCY OF TRANSGENIC RAT PRODUCTION&Thomas L.Saunders,Wanda E.Filipiak 45

0132 TRANSGENIC RABBITS AS MODELS FOR PRODUCING BIOLOGICALLY ACTIVE HUMAN RECOMBINANT PROTEINS&Hiripi L.,Baranyi M.等 46

0133 ESTABLISHMENT OF DOUBLE TRANSGENIC MICE MODEL SPATIOTEMPORALLY EXPRESSING HCV-C GENE MEDIATED BY TET-ON SYSTEM&CHEN Xi-Gu，YANG Guo-Shu等 47

0134 RE-STARVATION OF NUCLEUS-DONATING CELLS FOR GENE TRANSFER IN DAIRY GOATS ON EMBRYONIC DEVELOPMENT AFTER NUCLEAR TRANSPLANTATION（奶山羊转基因供核细胞的再饥饿对核移植胚胎发育的影响）&陈建泉，张爱民邓 47

0135 HUMAN PAPILLOMAVIRUS TRANSGENIC ANIMAL MODEL:STRATEGIES STILL TO BE IMPROVED（人乳头瘤病毒转基因动物模型：方法仍待改进） 49

0136 ESTABLISHMENT OF HIGH-COPY HBV TRANSGENIC MICE AND ITS APPLICATION（高复制HBV转基因小鼠建立及应用研究概况）&刘光泽，顾为望等 51

0137 SOMATIC CLONING AND MAMMARY GLAND BIOREACTOR（体细胞克隆与动物乳腺生物反应器）&SHI Qiao-Ting(施巧婷),XIN Xiao-Ling(辛晓玲)等 55

0138 PROSPECT OF THE ESTABLISHMENT OF TRANSGENIC ANIMALS BY PRIMITIVE SPERMATOGONIUM TRANSPLANTATION（用精原干细胞移植技术制作转基因动物的研究前景）&吴应积，罗奋华，旭日干 59

SECTION 2 GENE TARGETING（GENE KONCKOUT）（基因打靶／基因敲除） 62

0201 FSH,LH,E2 AND PIN BLOOD SERUM OF FMR1 KNOCKOUT MOUSE AND FVB MOUSE（雌性FMR1基因敲除小鼠血清生殖激素的比较研究）&DAI Li-Jun（戴丽军），LIAO Jun（廖军）等 62

0202 REGULATION OF DOPAMINE RECEPTORS ON COCAINE-INDUCED MAPK SIGNALING PATHWAY AND C-FOS GENE EXPRESSION（多巴胺受体在可卡因诱导的MAPK通路激活和C-FOS基因表达中的作用）&Liu Nu-Yun(刘怒云),Zhang Lu(张璐)等 65

0203 GENERATION OF THE CONDITIONAL GENE TARGETED MOUSE MODELS FOR HUMAN DISEASES&YANG Xiao 70

0204 GENE TARGETING WITH C57BL/6 STRAIN&Jingsong Chu，Illya Bronshteyn等 70

0205 MEKK1 REGULATES F-ACTIN AND EPITHELIUM MOVEMENT&LIU Nu-Yun,Zhang Lin等 71

0206 PRODUCTION OF KAPPA-CASEIN KNOCK-OUT ES CELL LINE DERIVED CHIMERAS&L.Hiripil,M.Baranyil等 72

0207 PRELIMINARY STUDY ON EFFECTS OF PASG KNOCKOUT ON BRAIN DEVELOPMENT AND RELEVANT GENE EXPRESSION（PASG基因敲除对脑发育及其相关基因表达影响的初步研究）&ZHANG Quan-Bin(张全斌),DONG Jun(董军)等 72

0208 OBSERVATIONAL STUDY ON BLEOMYCIN-INDUCED EXPRESSION OF A-ACTIN IN SMOOTH MUSCLE IN PULMONARY INTERSTITIAL FIBROSIS IN SMA-CRE/ROSA26 TRANSGENIC MICE（用SMA-CRE/ROSA26转基因鼠来观察博莱霉素诱导的肺间质纤维化中A平滑肌肌动蛋白的表达）&吴壮，蔡琳，徐军 73

0209 MOLECULAR AND CELLULAR BASIS OF DRUG ADDICTION（制备诱导性基因敲除小鼠研究安非他明依赖机制）&LI Sheng-Bin(李生斌) 74

SECTION 3　RNA INTERFERENCE（RNA干扰） 78

0301 RNA INTERFERENCE OF EXOGENOUS GFP GENE EXPRESSION IN NEURO-2A CELLS&ZHANG Xiao-Qin，LI Feng等 78

0302 SCREENING AND IDENTIFICATION OF SIRNA TO SUPPRESS MACROPHAGE MIGRATION INHIBITORY FACTOR（MIF）GENE EXPRESSION（抑制人巨噬细胞移动抑制因子（MIF）表达的SIRNA的筛选和鉴定）&SHAN Zhi-Xin（单志新），LIN Qiu-Xiong（林秋雄）等 81

0303 FABRICATION OF AN OLIGONUCLEOTIDE ARRAY FOR GENOME-WIDE MICRORNA AND EXPRESSION PROFILE OF MICRORNA IN HUMAN CANCER CELLS&MA Zhuo-Ya,LIU Min等 87

0304 GROWTH REPRESSION OF HUMAN CERVICAL CANCER HELA CELLS BY SIRNA TARGETING HUMAN TELOMERASE CATALYTIC SUBUNIT（HTERT）MRNA&LI Xin,SHEN Zhi-Wei等 87

0305 DOWN-REGULATION OF ALPHA-FETOPROTEIN（AFP）IN HEPG2 CELLS INTERFERED WITH SPECIFIC SIRNAAND ITS IMPACT ON THE CELLULAR FUNCTION&TANG Xiao-Yan，LI Xin等 88

0306 MIRNA:BIOGENESIS AND FUNCTION&TANG Hua 88

SECTION 4 EMBRYO ENGINEERING AND NUCLEAR TRANSFER（胚胎工程，核移植） 90

0401 IN VITRO DEVELOPMENT OF RECONSTRUCTED EMBRYO BY NUCLEAR TRANSFER WITH PORCINE SOMATIC CELLS（猪体细胞核移植重构胚的体外发育）&ZHENG De-Fu（张德福），Wang Y Ying（王英） 90

0402 PRODUCTION OF SHEEP TRANSGENIC EMBRYOS DERIVED FROM TRANSFECTED SOMATIC CELLS WITH HOMOLOGOUS RECOMBINANT VECTORS（利用同源重组载体生产转基因体细胞克隆绵羊胚胎）&AO Hong（敖红），PAN Qiu-Zhen（潘求真）等 95

0403 REMOVAL OF NECROTIC BLASTOMERES FROM HUMAN CRYOPRESERVED EMBRYOS ON PREGNANCY OUTCOME&LIU Wei-Xin，LUO Meng-Jun等 100

0404 INTEGRATION AND EXPRESSION OF DOUBLE SCREENING MARKER VECTOR IN DAIRY GOAT FETAL FIBROBLASTS（双元选择标记载体在奶山羊胎儿成纤维细胞的整合与表达）&李文蓉，谭立新等 106

0405 SCAVENGE STUDY OF THE FRESH EMBRYO ON EMBRYO TRANSFER IN SPECIFIC MUTANT MICE（采用鲜胚对特种突变小鼠进行胚胎移植的净化研究）&李瑞生，仇志华等 111

0406 CRYOPRESERVED MORULAE CAN BE USED TO EFFICIENTLY GENERATE GERMLINE-TRANSMITTING CHIMERAS BY BLASTOCYST INJECTION&Janice V.Parker-Thomburg,Jennifer L.Alana等 113

0407 STEM CELL AND THERAPEUTIC CLONING&ZHOU Qi 114

0408 EFFECTS OF DIFFERENT CHEMICAL ACTIVATION PROTOCOLS ON EARLY PARTHENOGENETIC DEVELOPMENT OF PORCINE OOCYTES&LU Sheng-Sheng，WEI Feng等 114

0409 IN VITRO PRODUCTION OF CATTLE EMBRYOS DERIVED FROM ABATTOIR OVARIES&ZHANG De-Fu，WANG Ying等 115

0410 STUDY ON THE OVUM PICK-UP BY ULTRASOUND-GUIDED RETRIEVAL AND IN VITRO EMBRYO PRODUCTION IN BOVINE（奶牛活体采卵体外生产胚胎的研究）&WANG Yu-Hong（王玉红），CHEN Jing-Bo（陈静波）等 115

0411 STUDY ON THE TECHNIQUE OF VITRIFICATION OF PORCINE OOCYTES&ZHANG De-Fu，LIU Dong等 117

0412 PROGRESS ON THE STUDY OF DIFFERENTIAL MEMBRANE PROTEINS BETWEEN XY CHROMOSOME BEARING SPERMATOZOA IN MAMMAL（哺乳动物X、Y精子间差异膜蛋白的研究进展）&WANG Dong（王栋），CHENG Jin-Hua（程金华）等 117

0413 GENETIC ENGINEERED ANIMALS AND CONTEMPORARY CCOMPARATIVE MEDICINE（基因工程动物与现代比较医学研究）&顾为望 刘光泽 王万山 121

SECTION 5 STEM CELLS（干细胞） 128

0501 THE ESTABLISHMENT OF PTET-ON INTEGRATED EMBRYONIC STEM CELL LINE OF MICE（稳定整合PTET-ON载体小鼠胚胎干细胞株的建立）&ZHANG Mei-Ying（张梅英），LI Hua（李华）等 128

0502 INDUCTION AND DIFFERENTIATION OF HEPATIC STEM CELLS INTO INSULIN-PRODUCING CELLS（肝干细胞诱导分化为胰岛素分泌细胞的实验研究）&AN Jing（安靓），SUN Bing（孙冰）等 133

0503 STEM CELLS ANDAIDS（干细胞技术与艾滋病研究）&XIONG Ya-Long（熊亚隆） 136

0504 TRANSCRIPTOMIC ANALYSIS OF THE CHICKEN EMBRYONIC STEM CELLS（CESC）&B.Pain 136

0505 IN VITRO ISOLATION,CULTIV-ATION AND IDENTIFICATION OF FETAL HEPATIC STEM CELLS（胎肝干细胞的分离、培养与鉴定）&SUN Bing（孙冰），AN Jing（安靓） 137

0506 IN UTERO TRANSPLANTATION OF HUMAN CORD BLOOD PRIMITIVE CELLS TRANSDUCED WITH MIG VECTOR INTO FETAL GOATS TO INVESTIGATE THE LONG-TERM GROWTH POTENTIAL AND PLASTICITY OF STEM CELLS&HUANG Shu-Zhen，ZENG Yi-Tao 138

0507 REPROGRAMMING SOMATIC CELL IN VITRO:NEW STRATEGY FOR ESTABLISHMENT OF PLURIPOTENT STEM CELLLINES（体细胞体外重编程：建立多能干细胞系的新策略）&LI Yu-Qiang(李裕强) 139

0508 BONE MARROW MESENCHYMAL STEM CELLS AND MYOCARDIAL TISSUE ENGINEERING（骨髓间充质干细胞与心肌组织工程）&YU Xi-Yong（余细勇），LI Xiao-Hong（李晓红） 144

SECTION 6 NEW ADVANCES IN TECHNOLOGY（新技术，新近展） 149

0601 CONSTRUCTION AND IDENTIFICATION OF HUMAN TISSUE KALLIKREIN GENE EUKARYOTIC EXPRESSING VECTOR&DAI Yong（戴勇） 149

0602 MURINE RESIDENT BONE MARROW MACROPHAGE PROMOTE ENUCLEATION OF TERMINALLY DIFFERENTIATED ERYTHROLEUKEMIA CELLS THROUGH DIRECT ATTACHMENT AND PHAGOCYTOSIS&KANG Jie，LIU Qian等 152

0603 STUDY OF THE GENE EXPRESSION PROFILE ON PIGR-TRANSFECTED TMNE CELLS TREATED WITH EBV AND TPA COOPERATIVELY（EBV与TPA协同处理转染人PIGR的TMNE细胞基因表达谱的研究）&WANG Shuang（王爽），LV Li-Chun（吕丽春） 156

0604 DETECTION OF GENETICCALLY MODIFIED COMPONENT IN FAMILIAR EDULIS FUNGI BY MULTIPLEX PCR（食用菌中转基因成分的多重PCR检测技术研究）&陈文炳，江树勋等 160

0605 STUDY ON THE IMMOBILIZATION OF SSDNA ON THE PT ELECTRODE（单链DNA在PT电极上的固定化研究）&YANG Min-Li（杨敏丽） 163

0606 ROLE OF VEGFR-3 SIGNALING IN TUMOR LYMPHANGIOGENESIS AND LYMPHATIC METASTASIS&Yulong He，Iiro Rajantie等 167

0607 INHIBITORY EFFECT OF CARVEDILOL ON CALCIUM CURRENT IN RAT VENTRICULAR MYOCYTES（卡维地洛对大鼠心室肌细胞L-型钙通道的抑制作用研究）&DENG Chun-Yu（邓春玉），YU Xi-Yong（余细勇） 168

0608 CHROMOSOMAL ANALYSIS OF BOVINE BLASTOCYSTS PRODUCED BY ICSI AND IVF WITH SORTED SPERMS&ZHANG Ming，LU Yang-Qing等 171

0609 RAPID HIGH-THROUGHPUT GENERATION OF PRECISE CUSTOM GENE MODIFICATIONS IN MICE USING THE VELOCIGENE SUITE OF TECHNOLOGIES&Nicholas W.Gale，Andrew J.Murphy等 172

0610 DEVELOPMENT-RELATED GENE EXPRESSION IN MOUSE PREIMPLANTATION EMBRYOS WITH DIFFERENT DEVELOPMENTAL POTENTIAL（不同发育潜力小鼠植入前胚胎中发育相关基因的表达）&LI Xiang-Ping，Y.Kato，Y.Tsunoda等 173

0611 APPLICATION AND PROSPECTS OF PARTICLE-MEDIATED GENE TRANSFER IN CANCER THERAPY（微粒介导的基因转移在癌症治疗中应用前景）&CHEN Jian-Quan（陈建海），PING Yuan（平渊） 173

0612 ADVANCES IN MAMMALIAN GENOMIC IMPRINTING（哺乳动物印迹基因研究进展）&Jiang MS(江明生),杨小淦等 174

0613 DEVELOPMENT OF GENE TRANSFECTION INSTRUMENT AND ITS APPLICATIONIN ANIMAL CELLS（基因脉冲导入仪的研制及在动物转基因技术上的应用）&SU Ming-Hao（苏明皓），XU Bao-Qiang（徐宝强）等 176

APPENDIX:Author Index 179