An Introduction To Paper Electrophoresis and Related MethodsPDF电子书下载

1．Introduction 1

Historical 1

Tiselius method 2

Scope of the Tiselius method 5

Terminology 7

References 8

2．Electrical Conduction of Solutions 10

Essentials of electrochemistry 10

Temperature variation of conductivity 11

Variation of conductivity with dilution 12

Independent mobility and absolute velocity of ions 14

3．Theoretical Considerations 17

Factors influencing the movement of particles in an electric field 17

Theory of paper electrophoresis 23

References 30

4．Technique of Paper Electrophoresis 31

Methods of supporting the filter paper 31

Other technical aspects 44

Preparative methods 46

References 49

5．Continuous Electrophoretic Separation 50

Principle 50

Apparatus 52

Some applications 57

References 60

6．Electrophoresis on the Cytoscale 61

7．Combination of Chromatography and Electrophoresis 63

Simultaneous chromatography and electrophoresis 63

Electrophoresis followed by chromatography 64

References 66

8．Organic Acids 67

Higher fatty acids 67

Keto acids 68

Di-and Tricarboxylic acids 68

References 69

9．Carbohydrates and Hydroxycompounds 70

Monosaccharides 71

Methyl ethers and disaccharides 73

Polysaccharides 75

Polyhydric alcohols and phenols 77

Flavonoids 79

References 80

10．Nitrogen Compounds 81

Amines 81

Aminosugars 81

Indole derivatives 81

Sulphanilamides 82

Amino acids and peptides 83

Two-dimensional separation of amino acids 86

DNP-amino acids 90

Guanido derivatives 89

Peptides 90

Iodothyronines and iodotyrosines 90

References 91

11．Alkaloids 92

12．Proteins 95

Details of the electrophoretic techniques 95

Quantitative evaluation of proteins on the paper 98

Detection of lipoids in serum fractions on the paper 106

Estimation of fibrinogen 108

Lysozyme 109

The subfractions of the serum globulins 109

Some clinical results 112

References 120

13．Nucleic Acids and Derivatives,and Phosphate Esters 125

Nucleic acids 125

Hydrolysis products of nucleic acids 126

Separation of ATP,ADP and related substances 130

Thiamine and thiamine phosphoric esters 132

Vitamin B2 and B6 groups 134

References 135

14．Antibiotics 136

15．Triterpenoids 138

16．Steroids 139

17．Dyes 141

18．Inorganic Compounds 143

Sequences of ions 143

Separations of analytical and preparative interest 147

References 155

19．Physico-chemical Studies 156

20．Electrophoresis in Packed Columns 162

Electrophoresis in a glass powder column 163

Electrophoresis in starch columns 164

Electrophoresis in a cellulose column 165

Electrophoresis in slabs of cellulose or starch 166

References 168

21．Electrophoresis Inside Gels 169

Electrophoresis in silica jelly 169

Electrophoresis in agar gel 174

References 186

22．Measurement of Diffusion Rates Inside Porous Media 187

23．Electrokinetic Ultrafiltration 189

Author Index 193

Subject Index 199