田波病毒学文选PDF电子书下载

第一篇 亚病毒 3

第一章 亚病毒的研究进展 3

亚病毒——病毒学的一个新分支 5

第二章 病毒卫星RNA及其在病毒病防治上的应用 13

黄瓜花叶病毒组的卫星RNA 15

植物病毒卫星RNA及其在病毒病生物防治上的应用 23

卫星RNA作为黄瓜花叶病毒的生防因子 26

卫星RNA作为黄瓜花叶病毒病生防因子的研究 30

植物病毒卫星RNA生防制剂CMV-S52防治番茄病毒病 36

烟台市应用弱毒疫苗S51和N14防治辣椒花叶病毒病的试验 38

带卫星RNA黄瓜花叶病毒保护接种的植物中病毒积累与基因组RNA合成及病状表达 40

抗黄瓜花叶病毒的卫星RNA互补DNA转基因番茄 47

Control of Two Seed-Borne Virus Diseases in China by The Use of Protective Inoculation 48

Double-Stranded Cucumovirus Associated RNA 5:Experimental Analysis of Necrogenic and Non-necrogenic Variants by Temperature-Gradient Gel Electrophoresis 52

Satellite RNA for The Biocontrol of Plant Disease 61

High Resistance to Cucumber Mosaic Virus Conferred by Satellite RNA and Coat Protein in Transgenic Commercial Tobacco Cultivar G-140 73

Resistance of Tomato Infected with Cucumber Mosaic Virus Satellite RNA to Potato Spindle Tuber Viroid 82

Viroid-Like RNA Encapsidated in Lucerne Transient Streak Virus 89

Multiplication of Velvet Tobacco Mottle Virus in Nicotiana clevelandii Protoplasts is Resistant to α-Amanitin 93

Plant Resistance to Fungal Diseases Induced by the Infection of Cucumber Mosaic Virus Attenuated by Satellite RNA 99

Rapid Production and Field Testing of Homozygous Transgenic Tobacco Lines with Virus Resistance Conferred by Expression of Satellite RNA and Coat Protein of Cucumber Mosaic Virus 104

Controlling Mosaic Virus Diseases Under Field Conditions Using Multiple Gene Strategies in Transgenic Plants 113

第三章 类病毒的研究 127

类病毒研究进展和展望 129

用互补DNA分子杂交和聚丙烯酰胺凝胶电泳测定我国菊花矮化类病毒 138

苹果锈果病组织中发现的环状类病毒RNA 142

我国菊花褪绿斑驳类病毒鉴定 143

苹果锈果病（ASSD）类病毒的侵染性 150

接种外表健康的梨树芽片诱发苹果锈果病类病毒 155

苹果锈果类病毒的温度梯度凝胶电泳 159

高抗马铃薯纺锤形块茎类病毒的转基因马铃薯细胞内核酶转录产物的分布 164

Pear as A Natural Host of Apple Scar Skin Viroid 168

Ribozyme-Mediated High Resistance Against Potato Spindle Tuber Viroid In Transgenic Potatoes 171

第四章 朊病毒的研究 179

朊病毒的研究进展 181

免疫印迹法检测牛海绵状脑病和羊瘙痒病 188

High-Level Expression and Secondary Structure Analysis of The Bovine Mature Prion Protein 193

Conformational Change of Glutathione-S-Transferase by Its Co-Expression with Prion Domain of Yeast Ure2p 197

On-Column Purification and Refolding of Recombinant Bovine Prion Protein:Using Its Octarepeat Sequences as A Natural Affinity Tag 203

An Engineered PrPsc-Like Molecule From The Chimera of Mammalian Prion Protein and Yeast Ure2p Prion-Inducing Domain 210

Interaction of Doppel with The Full-Length Laminin Receptor Precursor Protein 216

In vitro Self-Propagation of Recombinant PrPsc-Like Conformation Generated In The Yeast Cytoplasm 223

Bovine PrPc Directly Interacts with αβ-Crystalline 230

Aggregation of Prion Protein with Insertion Mutations is Proportional to The Number of Inserts 238

第二篇 植物病毒 253

第一章 病毒和高温对马铃薯花叶型退化的影响 253

马铃薯块茎发芽条件对芽内X-病毒浓度的影响 255

马铃薯在温度条件影响下对花叶病毒抵抗力的改变与种薯退化关系的证据 265

在马铃薯退化问题上耐病性变化观念的形成和验证 280

西藏高原的生态条件对于马铃薯花叶型退化的影响 284

病毒和高温在马铃薯花叶型退化中的作用 289

油乳剂对蚜虫传染非持久性植物病毒的抑制作用 296

Loss of Tolerance to Mosaic Viruses as The Cause of Potato Degeneration 303

Potato Degeneration Research in China 312

马铃薯种薯生产的研究Ⅰ．用茎尖培养方法生产马铃薯无病毒原种 316

马铃薯种薯生产的研究Ⅱ．几种血清学方法对马铃薯病毒X诊断效果的比较 322

马铃薯无病毒留种体系的研究 327

第二章 植物双生病毒 331

植物双生病毒研究进展 333

烟草曲叶双生病毒的研究Ⅰ．广西分离物的生物学特性和血清学反应 343

烟草曲叶双生病毒分子进化的初步研究 348

番茄曲叶病及其血清学和PCR测定 352

一种非洲番茄黄化曲叶病毒DNA的克隆 356

Chinese Squash Leaf Curl Virus:A New Whitefly-Transmitted Geminivirus 363

Chinese Tomato Yellow Leaf Curl Virus—A New Species of Geminivirus 370

第三章 植物病毒弱毒疫苗 377

植物病毒弱毒系及其应用Ⅰ．烟花叶病毒番茄株弱毒系的诱变和性质的研究 379

植物病毒弱株系及其应用Ⅱ．烟花叶病毒番茄株弱株系N11对番茄的保护作用 384

植物病毒弱株系及其应用Ⅲ．烟花叶病毒番茄株弱毒疫苗N14的安全性测定 388

浸根法对番茄接种弱毒株（N14）获得的效果 396

延边地区烟草病毒种类及病毒病的防治 398

油菜花叶病毒15号和菸花叶病毒间干扰作用的研究 405

用DNA杂交技术测定烟草花叶病毒与长叶车前花叶病毒的关系 411

番茄花叶病毒及其弱毒N14株RNAs在兔网织细胞裂解液体系中翻译产物的比较研究 415

相关和不相关病毒mRNA在兔网织细胞裂解液中的竞争性翻译 418

干扰素的功能表达机制 424

酵母校正tRNA的提取及其对番茄花叶病毒增殖的抑制作用 427

Taxonomy of Several Tobamoviruses from China as Determined by Molecular Hybridization Analysis with Complementary DNA 434

Classification of Several Tobamoviruses Isolated in China on the Basis of The Amino Acid Composition of Their Virion Proteins 440

Double-Stranded Viral RNA Content in Tobacco Leaves Infected with Virulent and Avirulent Isolates of Tomato Mosaic Virus 444

第四章 禾本科作物病毒及其克隆与表达 449

水稻条纹叶枯病毒分子生物学的研究——外壳蛋白分子量、氨基酸组成和N末端 451

小麦丛矮病毒的研究 454

虎尾草条纹花叶病毒DNA克隆对小麦的农杆菌侵染 461

水稻条纹叶枯病毒基因组含vRNA2 ORF片段的克隆、序列分析及其在原核中的表达 465

水稻普矮病毒基因组第十片段cDNA的克隆及其体外转录 469

水稻矮缩病毒大外膜蛋白基因的合成、克隆和序列分析 474

水稻矮缩病毒小外壳蛋白基因的合成、克隆和序列分析 478

Nucleotide Sequence of Maize Dwarf Mosaic Virus Capsid Protein Gene and Its Expression in Escherichia coli 482

Resistance to Rice Stripe Virus Conferred by Expression of Coat Protein in Transgenic Indica Rice Plants Regenerated from Bombarded Suspension Culture 488

The Study of Molecular Biology of Rice Stripe Virus（Ⅰ）——Molecular Weight,Amino Acid Composition and Property of Coat Protein 497

Infection of Barley Protoplasts with Barley Stripe Mosaic Virus Detected and Assayed by Immunoperoxidase 500

Ribozyme-Mediated Resistance to Rice Dwarf Virus and The Transgene Silencing in The Progeny of Transgenic Rice Plants 505

第五章 其他植物病毒及其克隆与表达 515

构建具侵染性的花椰菜花叶病毒克隆 517

芜青花叶病毒外壳蛋白基因的克隆和表达 521

番木瓜环斑病毒外壳蛋白基因的构建 524

一个黄瓜花叶病毒强株系的外壳蛋白基因的合成、克隆、序列分析和表达 528

哈密瓜花叶病流行的研究 533

引起哈密瓜坏死的两种病毒的分离及鉴定 537

油菜花叶病毒（YMV6）部分提纯及其若干性质的初步研究 543

用酶标抗体检测植物细胞和原生质体内的烟草花叶病毒 549

抗黄瓜花叶病毒单克隆抗体细胞株的建立及其抗体鉴定 555

用A蛋白夹层酶联免疫吸附法对黄瓜花叶病毒的血清学鉴定 561

Cucumber Mosaic Virus from Cornflower in China 566

Discovery and Demonstration of Small Circular DNA Molecules Derived from Chinese Tomato Yellow Leaf Curl Virus 570

第三篇 医学与动物病毒 577

第一章 乙肝病毒及其T细胞免疫与热休克蛋白96的佐剂作用 577

四环素调控表达系统调节EGFP和HBV前核心蛋白在哺乳动物细胞中表达 579

HBV-Specific Peptide Associated with Heat-Shock Protein gp96 585

Three-Step Purification of gp96 from Human Liver Tumor Tissues Suitable for Isolation of gp96-Bound Peptides 588

Significant Correlation Between Expression Level of Hsp gp96 and Progression of Hepatitis B Virus Induced Diseases 596

New Serum Biomarkers for Detection of HBV-Induced Liver Cirrhosis Using SELDI Protein Chip Technology 604

Generation of Murine CTL by A Hepatitis B Virus-Specific Peptide and Evaluation of the Adjuvant Effect of Heat Shock Protein Glycoprotein 96 and Its Terminal Fragments 609

Enhancement of Humoral Immune Responses to HBsAg By Heat Shock Protein gp96 and Its N-Terminal Fragment in Mice 622

Hepatitis B Virus Core Antigen Epitopes Presented by HLA-A2 Single-Chain Trimers Induce Functional Epitope-Specific CD8+ T-Cell Responses In HLA-A2.1/Kb Transgenic Mice 631

A Mutant HBs Antigen(HBsAg)183-191 Epitope Elicits Specific Cytotoxic T Lymphocytes in Acute Hepatitis B Patients 641

The High Prevalence of the 127 Mutant HBcAg 18-27 Epitope in Chinese HBV-Infected Patients and Its Cross-Reactivity with the V27 Prototype Epitope 650

Enhancing The potency of HBV DNA Vaccines Using Fusion Genes of HBV-Specific Antigens and the N-Terminal Fragment of gp96 660

PD-1 and PD-L1 Upregulation Promotes CD8+ T-Cell Apoptosis and Postoperative Recurrence in Hepatocellular Carcinoma Patients 678

Skewed Ratios Between CD3+ T Cells and Monocytes are Associated with Poor Prognosis in Patients with HBV-related Acute-on-chronic Liver Failure 690

第二章 其他医学与动物病毒 701

Following the Rule:Formation of the 6-Helix Bundle of the Fusion Core from Severe Acute Respiratory Syndrome Coronavirus Spike Protein and Identification of Potent Peptide Inhibitors 703

Six-Helix Bundle Assembly and Analysis of the Central Core of Mumps Virus Fusion Protein 710

Both Heptad Repeats of Human Respiratory Syncytial Virus Fusion Protein are Potent Inhibitors of Viral Fusion 718

The Antibodies Directed Against N-Terminal Heptad-repeat Peptide of hRSV Fusion Protein and Its Analog-5-Helix Inhibit Virus Infection in vitro 727

Design and Characterization of Human Respiratory Syncytial Virus Entry Inhibitors 735

Analysis of a Point Mutation in H5N1 Avian Influenza Virus Hemagglutinin in Relation to Virus Entry into Live Mammalian Cells 744

Evaluation of Glu11 and Gly8 of The H5N1 Influenza Hemagglutinin Fusion Peptide in Membrane Fusion Using Pseudotype Virus and Reverse Genetics 755

A Dual Reporter Gene Based System to Quantitate the Cell Fusion of Avian Influenza Virus H5N1 766

Structural Characterization of Mumps Virus Fusion Protein Core 774

Rattonal Design of Highly Potent HIV-1 Fusion Inhibitory Proteins:Implication for Developing Antiviral Therapeutics 782

Screening for CD8 Cytotoxic T Lymphocytes Specific for Gag of Human Immunodeficiency Virus Type 1 Subtype B'Henan Isolate from China and Identification of Novel Epitopes Restricted by the HLA-A2 and HLA-A11 Alleles 790

Recombinant Protein of Heptad-Repeat HR212,A Stable Fusion Inhibitor with Potent Anti-HIV Action in vitro 800

Glycoprotein 96-Mediated Presentation of Human Immunodeficiency Virus Type 1(HIV-1)-Specific Human Leukocyte Antigen Class I-Restricted Peptide and Humoral Immune Responses to HIV-1 p24 811

Effect of RNA Oligonucleotide Targeting Foxo-1 on Muscle Growth in Normal and Cancer Cachexia Mice 821

Myostatin Antisense RNA-Mediated Muscle Growth in Normal and Cancer Cachexia Mice 831

A Retrovirus-Based System to Stably Silence GDF-8 Expression and Enhance Myogenic Differentiation in Human Rhabdomyosarcoma Cells 840

Inhibition of Atrogin-1/MAFbx Expression by Adenovirus-Delivered Small Hairpin RNAs Attenuates Muscle Atrophy in Fasting Mice 852

Circulating microRNAs in Hepatitis B Virus-Infected Patients 868

Identification of the Myelin Oligodendrocyte Glycoprotein as a Cellular Receptor for Rubella Virus 880

贺信 897

中国科学院病原微生物与免疫学重点实验室简介 899

后记 903