BIOTECHNOLOGY A LABORATORY SKILLS COURSEPDF电子书下载

Chapter 1: The Biotechnology Industry 2

What Is Biotechnology? 3

Who Uses Biotechnology? 5

Biotechnology Industry and Research 13

Governmental Regulation of Biotechnology 15

Industry Practices 17

Careers in Biotechnology 19

Chapter 1 Essay Questions 20

Additional Resources 21

Chapter 1 Vignettes 3

Bioethics Biotechnology: Good or Bad? 3

Biotech In The Real World: Bio-Rad: Then and Now 12

How To... Write an SOP 17

Careers In Biotech:Annabelle Tinoco, Quality Assurance Manager 18

Chapter 2: Laboratory Skills 22

Laboratory Safety 23

Laboratory Notebooks 25

Laboratory Equipment 26

Numerical Data 33

Significant Figures 33

Scientific Notation 34

Units of Measure 35

Preparing Solutions 35

Percent Solutions 35

Using Stock Solutions Given in Terms of “x” 36

Molar Solutions 37

Chapter 2 Essay Questions 39

Additional Resources 39

Chapter 2 Laboratory Activities 40

Activity 2.1 DNA Extraction and Precipitation 40

Activity 2.2 Pipetting 42

Activity 2.3 Kool-Aid Column Chromatography 46

Activity 2.4 Making Solutions 49

Activity 2.5 Titration 54

Activity 2.6 Writing a Standard Operating Procedure 56

Chapter 2 Extension Activities 56

Chapter 2 Vignettes 24

Bioethics: Waste Disposal 24

Biotech In The Real World: Who Invented Claritin? How Laboratory Notebooks Played Their Part 26

Careers In Biotech:Katie Dalpozzo, Laboratory Research Associate 27

How To... Use an Adjustable-Volume Micropipet 29

Chapter 3: Microbiology and Cell Culture 58

Microbiology and Cell Biology 59

Three Domains of Life 59

Microorganisms and History 59

Bacteria 61

Uses of Bacteria in Biotechnology 62

Culturing Bacteria in the Laboratory 63

Microbiological Techniques 64

Eukaryotic Cells 69

Uses of Eukaryotic Cells in Biotechnology 71

Eukaryotic Cell Culture 72

Chapter 3 Essay Questions 74

Additional Resources 74

Chapter 3 Laboratory Activities 75

Activity 3.1 Making Microbiology Media 75

Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test） 83

Activity 3.3 Microbes and Health:An Illustration of Koch's Postulates 86

Activity 3.4 Gram Staining 92

Activity 3.5 Quantifying Bacterial Numbers 95

Activity 3.6 Staining Eukaryotic Cells 98

Chapter 3 Extension Activities 100

Chapter 3 Vignettes 65

How To... Use Aseptic Technique to Transfer Bacteria 65

Careers In Biotech:Mihaela Sabau, Clinical Laboratory Scientist 67

Bioethics:Should Human Embryos Be Used for Research? 72

Biotech In The Real World: Artificial Life! 73

Chapter 4: DNA Structure and Analysis 102

Molecular Biology 103

DNA Structure 103

Recombinant DNA Technology 105

Restriction Enzymes 106

Ligases 107

Horizontal Agarose Gel Electrophoresis 108

Restriction Enzymes as Forensic Tools 112

Restriction Fragment Length Polymorphisms 112

Chapter 4 Essay Questions 113

Additional Resources 113

Chapter 4 Laboratory Activities 114

Activity 4.1 Restriction Site Prediction Using NEBcutter 114

Activity 4.2 Casting Agarose Gels 116

Activity 4.3 Dye Electrophoresis 119

Activity 4.4 Restriction Digestion and Analysis of Lambda DNA 121

Activity 4.5 Forensic DNA Fingerprinting 126

Activity 4.6 Plasmid Mapping 131

Chapter 4 Extension Activities 133

Chapter 4 Vignettes 104

Bioethics: Personal Genetic Information 104

Careers In Biotech: Charles Lo, Commercial Rotation Development Program Associate 106

How To... Set Up a Restriction Digest 108

Biotech In The Real World: Did the Baker Do It? 112

Chapter 5: Bacterial Transformation and Plasmid Purification 134

History of Bacterial Transformation and Plasmids 135

Plasmids: Structure and Function 135

Using Plasmids in Biotechnology 137

Transcriptional Regulation of Plasmids 138

Transforming Cells 140

Selection of Transformed Cells 142

Plasmid Purification 144

DNA Quantitation 147

Chapter 5 Essay Questions 149

Additional Resources 149

Chapter 5 Laboratory Activities 150

Activity 5.1 Bacterial Transformation with S3 Plasmid 150

Activity 5.2 Bacterial Transformation withpGLO Plasmid 155

Activity 5.3 Purification of S3 and pGLO Plasmids 160

Activity 5.4 DNA Quantitation 166

Chapter 5 Extension Activities 172

Chapter 5 Vignettes 136

Bioethics: A World without Antibiotics? 136

How To... Transform E. coli Using Electroporation 141

Biotech In The Real World:Plasmids and the Search for Energy 143

How To... Use a Microcentrifuge 146

Careers In Biotech: Denise Gangadharan,Acting Associate Director for Science 148

Chapter 6: The Polymerase Chain Reaction 174

Invention of PCR 175

What Is PCR? 175

PCR and DNA Replication 177

Thermal Cyclers 180

Types of PCR 181

PCR Optimization 183

Techniques Based on PCR 185

Real-World Applications of PCR 187

Chapter 6 Essay Questions 191

Additional Resources 191

Chapter 6 Laboratory Activities 192

Activity 6.1 STR PCR Analysis 192

Activity 6.2 GMO Detection by PCR 197

Activity 6.3 Detection of the Human PV92 Alu Insertion 204

Chapter 6 Extension Activities 211

Chapter 6 Vignettes 180

Careers In Biotech: Stan Hitomi,Math and Science Coordinator and Principal 180

How To... Program a Thermal Cycler 182

Biotech In The Real World: Biotech on the Pharm 188

Bioethics: Forensic DNA Databases—Is Your Privacy Protected? 189

Chapter 7: Protein Structure and Analysis 212

Protein Synthesis 213

Protein Structure 215

Proteins in Biology 217

Proteins in Biotechnology 218

Methods of Protein Analysis 220

Protein Quantitation 220

Protein Electrophoresis 222

Protein Chromatography 225

Bioinformatics 228

Chapter 7 Essay Questions 229

Additional Resources 229

Chapter 7 Laboratory Activities 230

Activity 7.1 Protein Quantitation Using the Bradford Assay 230

Activity 7.2 Size Exclusion Chromatography 234

Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography 237

Activity 7.4 SDS-PAGE of Fish Muscle 243

Activity 7.5 Biofuel Enzyme Assay 252

Activity 7.6 Exploring Bioinformatics with GFP 264

Chapter 7 Extension Activities 269

Chapter 7 Vignettes 218

Bioethics: Food or Fuel? 218

Careers In Biotech: Scott Chilton, Graduate Student 220

How To... Set Up a Vertical Electrophoresis System 224

Biotech In The Real World: Vaccines in Bananas 227

Chapter 8: Immunological Applications 270

The Immune System 271

Antibodies 272

Antibodies as Tools 273

Immunoassays 275

Enzyme-Linked Immunosorbent Assay （ELISA） 278

Western Blotting 280

Chapter 8 Essay Questions 285

Additional Resources 285

Chapter 8 Laboratory Activities 286

Activity 8.1 Ouchterlony Double Immunodiffusion Assay 286

Activity 8.2 Serum Antibody Detection by ELISA 288

Activity 8.3 Quantitative ELISA 291

Activity 8.4 Western Blotting 296

Chapter 8 Extension Activities 303

Chapter 8 Vignettes 277

How To... Select an Antibody 277

Bioethics: Treatment of HIV Positive People in the Developing World 282

Biotech In The Real World:Using FACS to Diagnose and Treat Cancer 284

Careers In Biotech:Amanda Williams, Clinical Lab Technician 285

Chapter 9: Research Projects 304

What Is Research? 305

Student Research Projects 307

Choosing a Research Project 310

Planning a Project 312

Experimental Design 313

Data Analysis 314

Communicating Research 316

Project Ideas 318

Research Tools and Techniques 319

Agricultural and Animal Research 322

Food Science 326

Humans and Health 328

Forensics 330

Energy and the Environment 331

Considerations and Tips for All Laboratory Projects 333

Chapter 9 Essay Questions 336

Additional Resources 336

Chapter 9 Vignettes 306

Careers In Biotech: Sophy Wong, HIV Specialist 306

Bioethics: The Case of Woo Suk Hwang 307

Biotech In The Real World: Abraham Lincoln High School Takes On the iGEM Challenge 311

How To... Read a Journal Article 313

Appendices 338

Appendix A: Fast Gel Protocol 338

Appendix B: Alternative Staining Methods for Agarose Gels 339

Appendix C:Using the SmartSpec Plus Spectrophotometer 341

Appendix D: Glossary 344

Appendix E: Laboratory Skills Assessment Rubric 351

Appendix F: Laboratory Notebook and Project Rubrics 372

Laboratory Notebook Rubric 372

Project Progress Rubric 373

Project Proposal Rubric 374

Presentation Content Rubric 375

Presentation Style Rubric 376

Appendix G: Trademarks and Legal Notices 377