基因克隆和DNA分析 英文版PDF电子书下载

Part 1:The Basic Principles of Gene Cloning and DNA Analysis　 1

1　Why Gene Cloning and DNA Analysis are Important　 3

Contents 7

Preface to the Fourth Edition　 7

Preface to the Third Edition　 9

Preface to the Second Edition　 10

Preface to the First Edition　 12

2 Vehicles for Gene Cloning:Plasmids and Bacteriophages　 14

3　Purification of DNA from Living Cells　 28

4　Manipulation of Purified DNA　 52

5　Introduction of DNA into Living Cells　 85

6　Cloning Vectors for E．coli 104

7 Cloning Vectors for Eukaryotes 130

8　How to Obtain a Clone of a Specific Gene　 156

9　The Polymerase Chain Reaction　 179

Part 2:The Applications of Gene Cloning and DNA Analysis in Research　 195

10　Studying Gene Location and Structure　 197

11　Studying Gene Expression and Function　 217

12　Studying Genomes　 246

Part 3:The AppIications of Gene CIoning and DNA Analysis in Biotechnology　 269

13 Production of Protein from Cloned Genes 271

14 Gene Cloning and DNA Analysis in Medicine 293

15 Gene Cloning and DNA Analysis in Agriculture 313

16 Gene Cloning and DNA Analysis in Forensic Science 329

Glossary 341

Index 357