医学生物学实验 英文PDF电子书下载

Chapter 1 Introduction to the Medical Biology Laboratory 1

11 Objectives of a Teaching Medical Biology Laboratory 3

12 Laboratory Rules 3

13 Laboratory Safety 4

14 Common Laboratory Equipments 5

15 Experimental Methods in Medical Biology 5

16 Evaluation of Experimental Data and Laboratory Reports 18

Chapter 2 Experiments in Medical Biology 21

Experiment 1 The Use of Glass Pipets and Pipettors 23

Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins 27

Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay 29

Experiment 4 Quantitative Determination of Proteins by Folin-phenol Assay 32

Experiment 5 Determination of Proteins by UV Absorption 35

Experiment 6 Separation of Amino Acid Mixtures by Ion-exchange Chromatography 37

Experiment 7 Separation of Serumγ-globulins by Ion-exchange Chromatography 40

Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis 44

Experiment 9 Separation and Purity Determination of Serumγ-globulins by SDS-polyacrylamide Gel Electrophoresis 47

Experiment 10 Determination of Serum Glutamic-pyruvic Transaminase Activity 52

Experiment 11 Determination of Serum Urea Nitrogen Content 55

Experiment 12 Determination of Blood Glucose Concentration 58

Experiment 13 Subculture of Adhesion Cells 62

Experiment 14 Counting and Determination of Live/Dead Cells 64

Experiment 15 Preparation ofCell Samples for Observation of Cell Morphologies 67

Experiment 16 Mitosis and Meiosis of Cells 70

Experiment 17 Intracellular Distribution of Biomacromolecules 73

Experiment 18 Isolation of Subcellular Components 77

Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells 80

Experiment 20 Banding Pattern of Human Chromosomes 83

Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells 86

Experiment 22 Isolation,Amplification and Identification of the β-actin Gene Fragment from Mouse Liver Cells 91

Experiment 23 Purification of Target DNA and Generation of Recombinant DNA 97

Experiment 24 Transformation and Screening of Recombinant DNA 101

Experiment 25 Determination of Serum Proteins Using Western Blotting 106