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PART Ⅰ ISOLATION OF DENDRITIC CELLS FROM ANIMAL TISSUES 3

1 Isolation of Dendritic Cells from Mouse Lymph Nodes&Dmitry Gabrilovich 3

2 Isolation of Mouse Spleen Dendritic Cells&Andrew J. Stagg, Fiona Burke, Suzanne Hill,and Stella C. Knight 9

3 Isolation of Mouse Thymic Dendritic Cells&Fabienne Anjuère and Carlos Ardavin 23

4 Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen&Gordon MacPherson, Michelle Wykes, Fang-Ping Huang,and Chris Jenkins 29

5 Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells&Franz Koch, Eckhart K？mpgen, Gerold Schuler,and Nikolaus Romani 43

6 Murine Intestinal Dendritic Cell Isolation&Paul Pavli 63

7 Identification and Isolation of Rodent Lung Dendritic Cells&Philip A. Stumbles, Jennifer.Thomas, and Patrick G. Holt 73

8 Isolation and Propagation of Mouse Liver-Derived Dendritic Cells&Lina Lu, Maureen L. Drakes, and Angus W. Thomson 85

PART Ⅱ ISOLATION AND CHARACTERIZATION OF HUMAN DENDRITIC CELLS 99

9 Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells&Stephen P. Robinson 99

10 Purification of Dendritic Cells from Peripheral Blood&Steven Patterson, Aaron Rae, and Heather Donaghy 111

11 Isolation of Human Tonsillar Dendritic Cells&Paul U. Cameron and Gianna Stent 121

12 Isolation of Dendritic Cells from Human Afferent Lymph&Christoph U. Brand, Nikhil Yawalkar, Robert E. Hunger,and Lasse R. Braathen 137

13 Isolation of Human Skin Dendritic Cells by In Vitro Migration&Cornelia D. Richters, Matthias J. Hoekstra, John S. du Pont,Robert W. Kreis, and Eduard W. A. Kamperdijk 145

14 Human Intestinal Dendritic Cell Isolation&Paul Pavli 155

15 Isolation of Human Lung Dendritic Cells&Michel M. van den Heuvel, Carin E. G. Havenith,and Robert H. J. Beelen 163

16 Identification and Isolation of Synovial Dendritic Cells&Allison R. Pettit, Lois Cavanagh, and Ranjeny Thomas 175

PART Ⅲ DERIVATION OF ANIMAL DENDRITIC CELLS FROM PROGENITOR CELLS 191

17 Generation of Murine Bone-Marrow-Derived Dendritic Cells&Asher Maroof 191

18 Generation of Dendritic Cells from Rat Bone Marrow&Timothy Powell, James R. Major, and Gordon MacPherson 199

19 Derivation of Murine Dendritic Cells from Thymic Precursors&Karen Lucas, Dolores Saunders, and Li Wu 207

PART Ⅳ GENERATION OF MURINE DENDRITIC CELL LINES 219

20 Generation of Mouse Dendritic Cell Lines&Stefania Citterio, Maria Rescigno, Maria Foti,Francesca Granucci, Malgosia Matyszak,Giampiero Girolomoni, and Paola Ricciardi-Castagnoli 219

21 Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin&Thilo Jakob and Mark C. Udey 231

22 Derivation of Dendritic Cell Lines from Mouse Skin&Akira Takashima and Paul R. Bergstresser 243

PART Ⅴ GENERATION OF HUMAN DENDRITIC CELLS FROM PROGENITOR CELLS 257

23 Propagation of Human Dendritic Cells In Vitro&Christophe Caux and Bertrand Dubois 257

24 Generation of Dendritic Cells from Lymphoid Precursors&Anne Galy 275

25 Dendritic Cell Generation from Highly Purified CD14+ Monocytes&Winfried F. Pickl, Otto Majdic, and Walter Knapp 283

26 Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy&Michel F. Gilliet and Fank O. Nestle 297

PART Ⅵ ASPECTS OF DENDRITIC CELL FUNCTION 307

27 Chemotaxis of In Vitro Cultured Human Dendritic Cells&Walter Luini, Giovanna D'Amico, Sergio Bernasconi,Paola Allavena, Alberto Mantovani, and Silvano Sozzani 307

28 Adhesion of Dendritic Cells to Endothelia&K. Alun Brown 313

29 Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells&Giancarlo Bianchi, Giovanna D'Amico, Silvano Sozzani,Alberto Mantovani, and Paola Allavena 325

30 In Vivo Assays of Langerhans Cell Migration&Marie Cumberbatch, Rebecca J. Dearman, and lan Kimber 331

31 In Vitro Migration of Rat Dendritic Cells&Kenjiro Matsuno and Taichi Ezaki 347

32 Antigen Uptake by Dendritic Cells&Christophe Caux and Bertrand Dubois 369

33 Uptake of Antigen-Antibody Complexes by Human Dendritic Cells&Neil A. Fanger, Paul M. Guyre, and Robert F. Graziano 377

34 Immunoelectron Microscopy of Antigen Processing in Dendritic Cells&Monique J. Kleijmeer, George Posthuma, and Hans J. Geuze 387

35 Purification and Characterization of MHC Class Ⅱ Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Ceils&Philippe Pierre 413

36 Detection of Intracellular Cytokines in Dendritic Cells&Peter Kelleher 423

37 Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells&Wenru Song and Ronald G. Crystal 429

38 Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells&Xiaoli Huang, Zheng Fan, Lian Zheng,and Charles R. Rinaldo, Jr 441

Index 455