实验一 光学显微镜的结构和使用 1
Experiment 1 The Structure and the Usage of an Optical Microscope 5
实验二 细胞形态和细胞器的观察 10
Experiment 2 Observation of Cell Morphology and Organelles 13
实验三 显微测量 16
Experiment 3 Microscopic Measurement 18
实验四 细胞膜的通透性 20
Experiment 4 Permeability of Cell Membrane 22
实验五 聚乙二醇（PEG）诱导的细胞融合 25
Experiment 5 Cell Fusion Induced by Polyethylene Glycol（PEG） 27
实验六 线粒体的活体染色 29
Experiment 6 Supravital Staining of Mitochondria 31
实验七 动植物细胞骨架的制备和观察 33
Experiment 7 The Preparation and Observation of Cytoskeleton of Animal Cells and Plant Cells 35
实验八Feulgen染色法测定DNA 38
Experiment 8 Identify DNA by Feulgen Staining 40
实验九 毛囊细胞核仁组织区银染 43
Experiment 9 Silver Staining Technique for Nucleolus Organizer Region of Hair Follicle Cells 45
实验十 人类染色体标本制备 48
Experiment 10 Human Chromosome Preparation 50
实验十一 人类染色体常规核型分析 52
Experiment 11 Analysis of Conventional Human Chromosome Karyotype 55
实验十二动、植物细胞有丝分裂的观察 59
Experiment 12 Observation of Mitosis in Animal Cells and Plant Cells 61
实验十三 蝗虫精巢减数分裂的观察 64
Experiment 13 Observation of Meiosis in the Grasshopper Testis 67
实验十四 动物细胞原代培养 71
Experiment 14 Primary Culture of Animal Cells 73
实验十五 细胞的传代培养 75
Experiment15Cell Subculture 77
实验十六 细胞计数和死活细胞的鉴定 80
Experiment 16 Cell Counting and Identification of Dead and Alive Cells 83
实验十七MTT和CCK-8检测法 86
Experiment 17 MTT and CCK-8Detection 89
实验十八 植物愈伤组织的诱导 93
Experiment 18 Induction of Plant Callus 96
实验十九TRIzol法提取RNA和DNA 100
Experiment 19 RNA and DNA Extraction Using TRIzol Reagent 103
实验二十 琼脂糖电泳法鉴定DNA 106
Experiment 20 Identify DNA with the Method of Agarose Gel Electrophoresis 108
实验二十一 聚合酶链式反应（PCR） 110
Experiment 21 Polymerase Chain Reaction（PCR） 113
实验二十二 脂质体介导转染法 116
Experiment 22 Liposome-mediated Transfection 118
实验二十三DNA重组技术 120
Experiment 23 DNA Recombination Technology 125
实验二十四 蛋白质聚丙烯酰胺凝胶电泳及western blot分析 130
Experiment 24 Polyacrylamide Gel Electrophoresis And Western Blot for Proteins 134