全国高等学校教材 细胞生物学实验教程 双语版PDF电子书下载

实验一 光学显微镜的结构和使用 1

Experiment 1 The Structure and the Usage of an Optical Microscope 5

实验二 细胞形态和细胞器的观察 10

Experiment 2 Observation of Cell Morphology and Organelles 13

实验三 显微测量 16

Experiment 3 Microscopic Measurement 18

实验四 细胞膜的通透性 20

Experiment 4 Permeability of Cell Membrane 22

实验五 聚乙二醇（PEG）诱导的细胞融合 25

Experiment 5 Cell Fusion Induced by Polyethylene Glycol（PEG） 27

实验六 线粒体的活体染色 29

Experiment 6 Supravital Staining of Mitochondria 31

实验七 动植物细胞骨架的制备和观察 33

Experiment 7 The Preparation and Observation of Cytoskeleton of Animal Cells and Plant Cells 35

实验八Feulgen染色法测定DNA 38

Experiment 8 Identify DNA by Feulgen Staining 40

实验九 毛囊细胞核仁组织区银染 43

Experiment 9 Silver Staining Technique for Nucleolus Organizer Region of Hair Follicle Cells 45

实验十 人类染色体标本制备 48

Experiment 10 Human Chromosome Preparation 50

实验十一 人类染色体常规核型分析 52

Experiment 11 Analysis of Conventional Human Chromosome Karyotype 55

实验十二动、植物细胞有丝分裂的观察 59

Experiment 12 Observation of Mitosis in Animal Cells and Plant Cells 61

实验十三 蝗虫精巢减数分裂的观察 64

Experiment 13 Observation of Meiosis in the Grasshopper Testis 67

实验十四 动物细胞原代培养 71

Experiment 14 Primary Culture of Animal Cells 73

实验十五 细胞的传代培养 75

Experiment15Cell Subculture 77

实验十六 细胞计数和死活细胞的鉴定 80

Experiment 16 Cell Counting and Identification of Dead and Alive Cells 83

实验十七MTT和CCK-8检测法 86

Experiment 17 MTT and CCK-8Detection 89

实验十八 植物愈伤组织的诱导 93

Experiment 18 Induction of Plant Callus 96

实验十九TRIzol法提取RNA和DNA 100

Experiment 19 RNA and DNA Extraction Using TRIzol Reagent 103

实验二十 琼脂糖电泳法鉴定DNA 106

Experiment 20 Identify DNA with the Method of Agarose Gel Electrophoresis 108

实验二十一 聚合酶链式反应（PCR） 110

Experiment 21 Polymerase Chain Reaction（PCR） 113

实验二十二 脂质体介导转染法 116

Experiment 22 Liposome-mediated Transfection 118

实验二十三DNA重组技术 120

Experiment 23 DNA Recombination Technology 125

实验二十四 蛋白质聚丙烯酰胺凝胶电泳及western blot分析 130

Experiment 24 Polyacrylamide Gel Electrophoresis And Western Blot for Proteins 134