Part 1:The Basic Principles of Gene Cloning and DNA Analysis 1
1 Why Gene Cloning and DNA Analysis are Important 3
Contents 7
Preface to the Fourth Edition 7
Preface to the Third Edition 9
Preface to the Second Edition 10
Preface to the First Edition 12
2 Vehicles for Gene Cloning:Plasmids and Bacteriophages 14
3 Purification of DNA from Living Cells 28
4 Manipulation of Purified DNA 52
5 Introduction of DNA into Living Cells 85
6 Cloning Vectors for E．coli 104
7 Cloning Vectors for Eukaryotes 130
8 How to Obtain a Clone of a Specific Gene 156
9 The Polymerase Chain Reaction 179
Part 2:The Applications of Gene Cloning and DNA Analysis in Research 195
10 Studying Gene Location and Structure 197
11 Studying Gene Expression and Function 217
12 Studying Genomes 246
Part 3:The AppIications of Gene CIoning and DNA Analysis in Biotechnology 269
13 Production of Protein from Cloned Genes 271
14 Gene Cloning and DNA Analysis in Medicine 293
15 Gene Cloning and DNA Analysis in Agriculture 313
16 Gene Cloning and DNA Analysis in Forensic Science 329
Glossary 341
Index 357