Chapter 1 Introduction 1
Section 1 The contents and theories in Histochemistry 1
Section 2 The basic requirements of Histochemistry methods 4
Chapter 2 The Tissue Preparation 6
Section 1 Tissue collection 6
Section 2 Fixation 6
Section 3 Tissue washing-up,dehydration and clearance 15
Section 4 Embedding 17
Section 5 Sectioning 18
Section 6 Adherence and mounting 20
Section 7 Buffer 22
Chapter 3 The Carbohydrate and its Derivatives Histochemistry 24
Section 1 Classification 24
Section 2 Histochemistry methods 26
Chapter 4 The Nucleic Acid Histochemistry 33
Section 1 DNA Histochemical demonstration 33
Section 2 Comparison demonstration of DNA and RNA Histochemistry 35
Chapter 5 The Lipid Histochemistry 39
Section 1 Overview 39
Section 2 Demonstration of lipids with physical methods 40
Section 3 Demonstration of lipids with chemical methods 42
Chapter 6 Enzyme Histochemistry 44
Section 1 Enzyme and its basic histochemical theory 44
Section 2 Histochemistry for common enzymes 49
Chapter 7 The Basic Theory of Immunohistochemistry 54
Section 1 Basic Immunology 54
Section 2 Common markers and their detection 57
Section 3 Basic conditions 63
Chapter 8 Common Methods Used in Immunohistochemistry 74
Section 1 Principles 74
Section 2 Immunofluorescence method 76
Section 3 Immunoenzyme technique 80
Section 4 Avidin-biotin method 83
Section 5 Protein A method 87
Section 6 Immunogold method and immuno gold-silver method 89
Chapter 9 Specificity and Sensitivity of Immunohistochemistry 92
Section 1 Specificity and specific staining 92
Section 2 Control experiment 93
Section 3 The methods to enhance immunohistochemical sensitivity 95
Chapter 10 The Double-Staining in Immunohistochemistry 100
Section 1 Double-staining Immunohistochemistry on serial section 100
Section 2 Immunofluorescence double labeling technique 100
Section 3 Immunoenzyme double staining technique 102
Section 4 Immunoenzyme-Immunofluorescence double staining 109
Section 5 Immunoenzyme-Immunogold double staining 109
Chapter 11 The Lectin Histochemistry 111
Section 1 Characteristics and application of lectin 111
Section 2 Application of lectin Histochemistry 113
Chapter 12 The Progress of in Situ Display 119
Section 1 The envision method 119
Section 2 The catalyzed signal amplification method 119
Section 3 The ferric oxide alternative method for HRP 120
Chapter 13 In Situ Hybridization Immunohistochemistry 121
Section 1 Basic principle 121
Section 2 Probe preparation 121
Section 3 Procedure of in situ hybridization histochemistry 123
Section 4 Factors affecting in situ hybridization 138
Section 5 Control test 139
Chapter 14 In Situ Polymerase Chain Reaction Technique 143
Section 1 Basic principle 143
Section 2 Basic types 143
Section 3 Procedure 144
Section 4 Application of in situ PCR technology 147
Chapter 15 Electron Microscopic Cytochemical Technique 149
Section 1 Electron microscopic enzyme cytochemistry technology 149
Section 2 Electron microscopic Immunocytochemical technique 161
Chapter 16 The Quantitative Assay of Histochemistry Results 177
Section 1 The photomicrography 177
Section 2 The image analyzer 178
Section 3 The flow cytometry 180
Section 4 The laser scanning confocal microscopy 185
References 190
Appendix Ⅰ Preparation of Commonly Used Buffer 191
Appendix Ⅱ Histochemistry and Immunohistochemistry Experiments 194
Appendix Ⅲ Figures and pictures 223