现代组织化学技术和方法 英文PDF电子书下载

Chapter 1 Introduction 1

Section 1 The contents and theories in Histochemistry 1

Section 2 The basic requirements of Histochemistry methods 4

Chapter 2 The Tissue Preparation 6

Section 1 Tissue collection 6

Section 2 Fixation 6

Section 3 Tissue washing-up,dehydration and clearance 15

Section 4 Embedding 17

Section 5 Sectioning 18

Section 6 Adherence and mounting 20

Section 7 Buffer 22

Chapter 3 The Carbohydrate and its Derivatives Histochemistry 24

Section 1 Classification 24

Section 2 Histochemistry methods 26

Chapter 4 The Nucleic Acid Histochemistry 33

Section 1 DNA Histochemical demonstration 33

Section 2 Comparison demonstration of DNA and RNA Histochemistry 35

Chapter 5 The Lipid Histochemistry 39

Section 1 Overview 39

Section 2 Demonstration of lipids with physical methods 40

Section 3 Demonstration of lipids with chemical methods 42

Chapter 6 Enzyme Histochemistry 44

Section 1 Enzyme and its basic histochemical theory 44

Section 2 Histochemistry for common enzymes 49

Chapter 7 The Basic Theory of Immunohistochemistry 54

Section 1 Basic Immunology 54

Section 2 Common markers and their detection 57

Section 3 Basic conditions 63

Chapter 8 Common Methods Used in Immunohistochemistry 74

Section 1 Principles 74

Section 2 Immunofluorescence method 76

Section 3 Immunoenzyme technique 80

Section 4 Avidin-biotin method 83

Section 5 Protein A method 87

Section 6 Immunogold method and immuno gold-silver method 89

Chapter 9 Specificity and Sensitivity of Immunohistochemistry 92

Section 1 Specificity and specific staining 92

Section 2 Control experiment 93

Section 3 The methods to enhance immunohistochemical sensitivity 95

Chapter 10 The Double-Staining in Immunohistochemistry 100

Section 1 Double-staining Immunohistochemistry on serial section 100

Section 2 Immunofluorescence double labeling technique 100

Section 3 Immunoenzyme double staining technique 102

Section 4 Immunoenzyme-Immunofluorescence double staining 109

Section 5 Immunoenzyme-Immunogold double staining 109

Chapter 11 The Lectin Histochemistry 111

Section 1 Characteristics and application of lectin 111

Section 2 Application of lectin Histochemistry 113

Chapter 12 The Progress of in Situ Display 119

Section 1 The envision method 119

Section 2 The catalyzed signal amplification method 119

Section 3 The ferric oxide alternative method for HRP 120

Chapter 13 In Situ Hybridization Immunohistochemistry 121

Section 1 Basic principle 121

Section 2 Probe preparation 121

Section 3 Procedure of in situ hybridization histochemistry 123

Section 4 Factors affecting in situ hybridization 138

Section 5 Control test 139

Chapter 14 In Situ Polymerase Chain Reaction Technique 143

Section 1 Basic principle 143

Section 2 Basic types 143

Section 3 Procedure 144

Section 4 Application of in situ PCR technology 147

Chapter 15 Electron Microscopic Cytochemical Technique 149

Section 1 Electron microscopic enzyme cytochemistry technology 149

Section 2 Electron microscopic Immunocytochemical technique 161

Chapter 16 The Quantitative Assay of Histochemistry Results 177

Section 1 The photomicrography 177

Section 2 The image analyzer 178

Section 3 The flow cytometry 180

Section 4 The laser scanning confocal microscopy 185

References 190

Appendix Ⅰ Preparation of Commonly Used Buffer 191

Appendix Ⅱ Histochemistry and Immunohistochemistry Experiments 194

Appendix Ⅲ Figures and pictures 223