PART Ⅰ ISOLATION OF DENDRITIC CELLS FROM ANIMAL TISSUES 3
1 Isolation of Dendritic Cells from Mouse Lymph Nodes&Dmitry Gabrilovich 3
2 Isolation of Mouse Spleen Dendritic Cells&Andrew J. Stagg, Fiona Burke, Suzanne Hill,and Stella C. Knight 9
3 Isolation of Mouse Thymic Dendritic Cells&Fabienne Anjuère and Carlos Ardavin 23
4 Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen&Gordon MacPherson, Michelle Wykes, Fang-Ping Huang,and Chris Jenkins 29
5 Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells&Franz Koch, Eckhart K？mpgen, Gerold Schuler,and Nikolaus Romani 43
6 Murine Intestinal Dendritic Cell Isolation&Paul Pavli 63
7 Identification and Isolation of Rodent Lung Dendritic Cells&Philip A. Stumbles, Jennifer.Thomas, and Patrick G. Holt 73
8 Isolation and Propagation of Mouse Liver-Derived Dendritic Cells&Lina Lu, Maureen L. Drakes, and Angus W. Thomson 85
PART Ⅱ ISOLATION AND CHARACTERIZATION OF HUMAN DENDRITIC CELLS 99
9 Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells&Stephen P. Robinson 99
10 Purification of Dendritic Cells from Peripheral Blood&Steven Patterson, Aaron Rae, and Heather Donaghy 111
11 Isolation of Human Tonsillar Dendritic Cells&Paul U. Cameron and Gianna Stent 121
12 Isolation of Dendritic Cells from Human Afferent Lymph&Christoph U. Brand, Nikhil Yawalkar, Robert E. Hunger,and Lasse R. Braathen 137
13 Isolation of Human Skin Dendritic Cells by In Vitro Migration&Cornelia D. Richters, Matthias J. Hoekstra, John S. du Pont,Robert W. Kreis, and Eduard W. A. Kamperdijk 145
14 Human Intestinal Dendritic Cell Isolation&Paul Pavli 155
15 Isolation of Human Lung Dendritic Cells&Michel M. van den Heuvel, Carin E. G. Havenith,and Robert H. J. Beelen 163
16 Identification and Isolation of Synovial Dendritic Cells&Allison R. Pettit, Lois Cavanagh, and Ranjeny Thomas 175
PART Ⅲ DERIVATION OF ANIMAL DENDRITIC CELLS FROM PROGENITOR CELLS 191
17 Generation of Murine Bone-Marrow-Derived Dendritic Cells&Asher Maroof 191
18 Generation of Dendritic Cells from Rat Bone Marrow&Timothy Powell, James R. Major, and Gordon MacPherson 199
19 Derivation of Murine Dendritic Cells from Thymic Precursors&Karen Lucas, Dolores Saunders, and Li Wu 207
PART Ⅳ GENERATION OF MURINE DENDRITIC CELL LINES 219
20 Generation of Mouse Dendritic Cell Lines&Stefania Citterio, Maria Rescigno, Maria Foti,Francesca Granucci, Malgosia Matyszak,Giampiero Girolomoni, and Paola Ricciardi-Castagnoli 219
21 Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin&Thilo Jakob and Mark C. Udey 231
22 Derivation of Dendritic Cell Lines from Mouse Skin&Akira Takashima and Paul R. Bergstresser 243
PART Ⅴ GENERATION OF HUMAN DENDRITIC CELLS FROM PROGENITOR CELLS 257
23 Propagation of Human Dendritic Cells In Vitro&Christophe Caux and Bertrand Dubois 257
24 Generation of Dendritic Cells from Lymphoid Precursors&Anne Galy 275
25 Dendritic Cell Generation from Highly Purified CD14+ Monocytes&Winfried F. Pickl, Otto Majdic, and Walter Knapp 283
26 Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy&Michel F. Gilliet and Fank O. Nestle 297
PART Ⅵ ASPECTS OF DENDRITIC CELL FUNCTION 307
27 Chemotaxis of In Vitro Cultured Human Dendritic Cells&Walter Luini, Giovanna D'Amico, Sergio Bernasconi,Paola Allavena, Alberto Mantovani, and Silvano Sozzani 307
28 Adhesion of Dendritic Cells to Endothelia&K. Alun Brown 313
29 Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells&Giancarlo Bianchi, Giovanna D'Amico, Silvano Sozzani,Alberto Mantovani, and Paola Allavena 325
30 In Vivo Assays of Langerhans Cell Migration&Marie Cumberbatch, Rebecca J. Dearman, and lan Kimber 331
31 In Vitro Migration of Rat Dendritic Cells&Kenjiro Matsuno and Taichi Ezaki 347
32 Antigen Uptake by Dendritic Cells&Christophe Caux and Bertrand Dubois 369
33 Uptake of Antigen-Antibody Complexes by Human Dendritic Cells&Neil A. Fanger, Paul M. Guyre, and Robert F. Graziano 377
34 Immunoelectron Microscopy of Antigen Processing in Dendritic Cells&Monique J. Kleijmeer, George Posthuma, and Hans J. Geuze 387
35 Purification and Characterization of MHC Class Ⅱ Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Ceils&Philippe Pierre 413
36 Detection of Intracellular Cytokines in Dendritic Cells&Peter Kelleher 423
37 Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells&Wenru Song and Ronald G. Crystal 429
38 Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells&Xiaoli Huang, Zheng Fan, Lian Zheng,and Charles R. Rinaldo, Jr 441
Index 455