分子生物学 影印版PDF电子书下载

Section A-Cells and macromolecules 1

A1 Cellular classification 1

A2 Subcellular organelles 4

A3 Macromolecules 7

A4 Large macromolecular assemblies 11

Section B-Protein structure 15

B1 Amino acids 15

B2 Protein structure and function 18

B3 Protein analysis 25

Section C-Properties of nucleic acids 31

C1 Nucleic acid structure 31

C2 Chemical and physical properties of nucleic acids 36

C3 Spectroscopic and thermal properties of nucleic acids 40

C4 DNA supercoiling 43

Section D-Prokaryotic and eukaryotic chromosome structure 47

D1 Prokaryotic chromosome structure 47

D2 Chromatin structure 49

D3 Eukaryotic chromosome structure 54

D4 Genome complexity 59

Section E-DNA replication 63

E1 DNA replication:an overview 63

E2 Bacterial DNA replication 68

E3 Eukaryotic DNA replication 72

Section F-DNA damage,repair and recombination 77

F1 Mutagenesis 77

F2 DNA damage 81

F3 DNA repair 84

F4 Recombination 87

Section G-Gene manipulation 91

G1 DNA cloning:an overview 91

G2 Preparation of plasmid DNA 96

G3 Restriction enzymes and electrophoresis 99

G4 Ligation,transformation and analysis of recombinants 104

Section H-Cloning vectors 111

H1 Design of plasmid vectors 111

H2 Bacteriophage vectors 115

H3 Cosmids and YACs 120

H4 Eukaryotic vectors 124

Section I-Gene libraries and screening 129

I1 Genomic libraries 129

I2 cDNA libraries 132

I3 Screening procedures 137

Section J-Analysis and uses of cloned DNA 141

J1 Characterization of clones 141

J2 Nucleic acid sequencing 146

J3 Polymerase chain reaction 150

J4 Organization of cloned genes 154

J5 Mutagenesis of cloned genes 158

J6 Applications of cloning 162

Section K-Transcription in prokaryotes 165

K1 Basic principles of transcription 165

K2 Escherichia coli RNA polymerase 168

K3 The E.coliσ70 promoter 170

K4 Transcription initiation,elongation and termination 173

Section L-Regulation of transcription in prokaryotes 179

L1 The lac operon 179

L2 The trp operon 183

L3 Transcriptional regulation by alternativeσfactors 187

Section M-Transcription in eukaryotes 191

M1 The three RNA polymerases:characterizationand function 191

M2 RNA Pol Ⅰgenes:the ribosomal repeat 193

M3 RNA Pol Ⅲ genes:5S and tRNA transcription 197

M4 RNA Pol Ⅱ genes:promoters and enhancers 201

M5 General transcription factors and RNA Pol Ⅱ initiation 203

Section N-Regulation of transcription in eukaryotes 207

N1 Eukaryotic transcription factors 207

N2 Examples of transcriptional regulation 213

Section O-RNA processing and RNPs 219

O1 rRNA processing and ribosomes 219

O2 tRNA processing,RNase P and ribozymes 225

O3 mRNA processing,hnRNPs and snRNPs 228

O4 Alternative mRNA processing 234

Section P-The genetic code and tRNA 237

P1 The genetic code 237

P2 tRNA structure and function 241

Section Q-Protein synthesis 247

Q1 Aspects of protein synthesis 247

Q2 Mechanism of protein synthesis 251

Q3 Initiation in eukaryotes 257

Q4 Translational control and post-translational events 261

Section R-Bacteriophages and eukaryotic viruses 265

R1 Introduction to viruses 265

R2 Bacteriophages 269

R3 DNA viruses 274

R4 RNA viruses 278

Section S-Tumor viruses and oncogenesb 283

S1 Oncogenes found in tumor viruses 283

S2 Categories of oncogenes 287

S3 Tumor suppressor genes 290

Further reading 295

Index 299